🧫
16S rRNA sequencing of gut microbiome in PH-PS treated mice
active
experiment
Created: 2026-04-10T22:33:51
By: etl-v1-backfill
Quality:
50%
✓ SciDEX
ID: exp-5576747b-f72b-4daa-8e5c-c2b791a59ad8
🧫 Experiment Protocol
ExploratoryAlzheimer's disease16S rRNA5×FAD transgenic miceproposed
This experiment specifically focused on analyzing the gut microbiome changes induced by PH-PS treatment using 16S rRNA gene sequencing. The study examined the intestinal flora composition in 5×FAD mice before and after PH-PS treatment to understand how the polysaccharide modulates gut bacteria populations. The sequencing analysis revealed that PH-PS treatment promoted beneficial probiotic microbiota including Lactobacillus, Muribaculum, Monoglobus, and [Eubacterium]_siraeum_group, while simultaneously suppressing inflammation-related bacteria such as UCG-009 and Blautia. This microbiome remodeling was linked to the restoration of intestinal barrier function and reduction of peripheral inflammation. The experiment provided crucial evidence for the gut-brain axis mechanism underlying PH-PS neuroprotective effects, demonstrating that dietary polysaccharides can influence brain health through modulation of the intestinal microbiome.
PRIMARY OUTCOME
gut microbiome composition changes
EXPECTED OUTCOMES
Increased beneficial bacteria, decreased harmful bacteria, improved gut barrier function
SUCCESS CRITERIA
Promotion of probiotic microbiota (Lactobacillus, Muribaculum, Monoglobus, [Eubacterium]_siraeum_group) and suppression of inflammation-related bacteria (UCG-009, Blautia)
PROTOCOL
16S rRNA gene sequencing, bioinformatics analysis of bacterial taxa, correlation analysis with neuroinflammatory markers
LINKED HYPOTHESES
Source: PMID 38750854 ↗
🧫 Experiment Extras
PATHWAY
gut-brain axis, intestinal barrier function
MARKET PRICE
$0.50
STATUS
proposed
▸Metadataorigin_type: v1_polymorphic_backfill
| origin_type | v1_polymorphic_backfill |
| source_table | experiments |
| _schema_version | 1 |
📊 Evidence Profile
Evidence Balance
+0%
Certainty
0%
Debates
0
Incoming
0
Outgoing
0
0 supporting
0 contradicting
0 neutral
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