🧫
Fecal microbiota transplantation from periodontitis donors
active
experiment
Created: 2026-04-10T22:49:25
By: etl-v1-backfill
Quality:
50%
✓ SciDEX
ID: exp-fa0d03e3-261c-4eac-8f67-b02263a7a267
🧫 Experiment Protocol
ValidationPeriodontitis-associated gut dysbiosisGPR109AGerm-free mice with fecal microbiota transplantationproposed
This experiment utilized germ-free mice as recipients for fecal microbiota transplantation to directly test the role of periodontitis-associated gut microbiota in mediating intestinal dysfunction. Fecal microbiota from control mice (GF-CON) and ligature-treated mice with periodontitis (GF-LIG) were transplanted into germ-free recipients. The study then analyzed colonic GPR109A levels, tight junction integrity, and inflammatory responses in the recipient mice. This approach allowed for direct causal testing of whether the altered microbiota composition from periodontitis donors could recapitulate the intestinal pathology observed in the original periodontitis model, thereby confirming a microbiota-dependent mechanism.
PRIMARY OUTCOME
GPR109A suppression and barrier dysfunction in recipient mice
EXPECTED OUTCOMES
Microbiota from periodontitis donors would suppress GPR109A and induce barrier dysfunction
SUCCESS CRITERIA
Significant GPR109A suppression, tight junction impairment, and inflammatory upregulation in recipients of periodontitis microbiota
PROTOCOL
Fecal microbiota transplantation from control and periodontitis donor mice into germ-free recipients, followed by analysis of GPR109A expression, tight junction proteins, and inflammatory markers
Source: PMID 41816355 ↗
🧫 Experiment Extras
PATHWAY
Microbiota-GPR109A-intestinal barrier axis
MARKET PRICE
$0.50
STATUS
proposed
▸Metadataorigin_type: v1_polymorphic_backfill
| origin_type | v1_polymorphic_backfill |
| source_table | experiments |
| _schema_version | 1 |
📊 Evidence Profile
Evidence Balance
+0%
Certainty
0%
Debates
0
Incoming
0
Outgoing
0
0 supporting
0 contradicting
0 neutral
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