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PPP3/calcineurin-dependent TFEB dephosphorylation mechanism

experiment Created: 2026-04-06 12:28:58 By: experiment_extractor Quality: 80% ✓ SciDEX ID: experiment-exp-ad6850c9-f4ec-48ac-9df6-a
🧫 Experiment Protocol Protein Interaction
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Related Entities
PPP3CB
Metadata
experiment_typeprotein_interaction
protocolCalcium imaging, immunofluorescence microscopy, western blotting for phospho-TFEB, subcellular fractionation, phosphatase activity assays
expected_outcomesLysosomal calcium release would activate calcineurin, leading to TFEB dephosphorylation and nuclear translocation
success_criteriaDemonstration of calcium-dependent TFEB dephosphorylation and nuclear translocation that can be blocked by calcineurin inhibitors
sample_sizeNone
statistical_methodsNone
p_valueNone
effect_sizeNone
extracted_at2026-04-06T05:28:58.489681
source_pmid30335591
_origin{'type': 'internal', 'url': None, 'tracked_at': '2026-04-06T12:28:58.521361'}
🌍 Provenance Graph 2 nodes, 1 edges

derives from (1)

Linked Artifacts (29)
derives_from📄Trehalose induces autophagy via lysosomal-mediated TFEB acti100%
mentions📖PPP3CB Gene80%
mentions📖PPP3CB Protein80%
related📖PPP3CB Gene72%
related📖PPP3CB Protein72%
mentions📖entities-tfeb70%
mentions📖TFEB70%
mentions📖TFEB-Mediated Lysosomal Biogenesis70%
mentions📖TFEB Activators for Parkinson's Disease70%
mentions📖TFEB Autophagy Pathway70%
mentions📖TFEB Signaling in Neurodegeneration70%
mentions📖TFEB Protein (Transcription Factor EB)70%
mentions📖TFEB Protein70%
mentions📖TFEB Activator Therapies for Neurodegenerative Diseases70%
mentions📖TFEB Activators in Neurodegeneration70%
mentions📖tfeb-activators-neurodegeneration70%
related📖entities-tfeb63%
related📖TFEB63%
related📖TFEB-Mediated Lysosomal Biogenesis63%
related📖TFEB Activators for Parkinson's Disease63%
related📖TFEB Autophagy Pathway63%
related📖TFEB Signaling in Neurodegeneration63%
related📖TFEB Protein (Transcription Factor EB)63%
related📖TFEB Protein63%
related📖TFEB Activator Therapies for Neurodegenerative Diseases63%
related📖TFEB Activators in Neurodegeneration63%
related📖tfeb-activators-neurodegeneration63%
mentions📖researchers60%
related📖researchers54%