🧫
Germfree mouse colonocyte energy metabolism analysis
active
experiment
Created: 2026-04-10T23:13:52
By: experiment_extractor
Quality:
90%
✓ SciDEX
ID: experiment-exp-cf95a475-75fb-403f-9b98-f
🧫 Experiment Protocol
Animal Modelproposed
▸Metadata
| _origin | {'url': None, 'type': 'internal', 'tracked_at': '2026-04-10T23:13:52.204066'} |
| p_value | None |
| protocol | Comparison of metabolic parameters between germfree and conventional mouse colonocytes, including enzyme expression analysis, NADH/NAD+ ratio measurement, ATP quantification, AMPK and p27(kip1) phosph |
| effect_size | None |
| sample_size | None |
| source_pmid | 21531334 |
| extracted_at | 2026-04-10T16:13:52.199462 |
| _schema_version | 1 |
| experiment_type | animal_model |
| success_criteria | Demonstration of decreased metabolic enzyme expression, reduced energy production, and activation of energy-sensing pathways in germfree colonocytes |
| expected_outcomes | Germfree colonocytes would show signs of energy deprivation due to lack of bacterial butyrate |
| statistical_methods | None |
📊 Evidence Profile
Foundational
Evidence Balance
+0%
Certainty
100%
Debates
0
Incoming
28
Outgoing
27
0 supporting
0 contradicting
0 neutral
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