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Ex vivo IGF-I treatment in rd10 organotypic retinal explants

experiment Created: 2026-04-10 22:34:11 By: experiment_extractor Quality: 90% ✓ SciDEX ID: experiment-exp-da891eb0-f020-4272-b989-5
🧫 Experiment Protocol Animal Model
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Pde6b
Metadata
experiment_typeanimal_model
protocolOrganotypic retinal explants treated with IGF-I, cell death assessed by TUNEL staining and ELISA of free nucleosomes, microglial visualization by Cd11b and Iba1 immunolabeling, microglial depletion wi
expected_outcomesIGF-I treatment would reduce photoreceptor cell death and modulate microglial response
success_criteriaReduction in TUNEL-positive nuclei and free nucleosome levels
sample_sizeNone
statistical_methodsNone
p_valueNone
effect_sizeNone
extracted_at2026-04-10T15:34:11.650907
source_pmid22039242
_origin{'type': 'internal', 'url': None, 'tracked_at': '2026-04-10T22:34:11.654907'}
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Linked Artifacts (17)
related🧫Microglial depletion study using clodronate liposomes100%
related🧫In vivo IGF-I intravitreal injection in rd10 mice100%
mentions📖PDE6B — Phosphodiesterase 6B80%
related📖PDE6B — Phosphodiesterase 6B72%
mentions📖IGF-1 Receptor Neurons70%
mentions📖IGF-1 Signaling Pathway in Neurodegeneration70%
related📖IGF-1 Receptor Neurons63%
related📖IGF-1 Signaling Pathway in Neurodegeneration63%
mentions📖Retinitis Pigmentosa60%
mentions📖Glial Cells60%
mentions📖Microglia60%
mentions📖Microglia60%
related📖Retinitis Pigmentosa60%
related📖Retinitis Pigmentosa54%
related📖Glial Cells54%
related📖Microglia54%
related📖Microglia54%