🧫

P2RY12 regulation of autophagy in VSMCs

experiment Created: 2026-04-04 05:26:15 By: experiment_extractor Quality: 90% ✓ SciDEX ID: experiment-exp-f90ceea7-76be-4e57-80d0-9
🧫 Experiment Protocol Cell Biology
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Related Entities
P2RY12, ATG5
Metadata
experiment_typecell_biology
protocolWestern blot for LC3 and SQSTM1, transmission electron microscopy, ATG5 knockdown, autophagosome quantification
expected_outcomesP2RY12 activation would inhibit autophagy markers and autophagosome formation
success_criteriaDecreased LC3-II/LC3-I ratio, reduced SQSTM1 degradation, fewer autophagosomes
sample_sizeNone
statistical_methodsNone
p_valueNone
effect_sizeNone
extracted_at2026-04-03T22:26:15.619135
source_pmid32160082
_origin{'type': 'internal', 'url': None, 'tracked_at': '2026-04-04T05:26:15.734801'}
🌍 Provenance Graph 2 nodes, 1 edges

derives from (1)

Linked Artifacts (93)
derives_from📄The P2RY12 receptor promotes VSMC-derived foam cell formatio100%
experiment_target📖P2RY12100%
mentions📖ATG5100%
mentions📖P2RY12100%
mentions📖ATG5 — Autophagy Related 580%
related📖ATG5 — Autophagy Related 572%
mentions📖ATG5 Protein70%
mentions📖ATG5 — ATG5 Protein70%
mentions📖LC3 (MAP1LC3) Neurons70%
mentions📖MAP1LC3A Gene70%
mentions📖LC3 Protein70%
mentions📖LC3 (Microtubule-Associated Protein 1 Light Chain 3)70%
mentions📖P2RY12 Gene70%
mentions📖SQSTM1/p6270%
mentions📖SQSTM1 — Sequestosome 1 (p62)70%
mentions📖SQSTM1 Protein (p62/Sequestosome-1)70%
mentions📖SQSTM1/p62 Protein70%
related📖ATG5 Protein63%
related📖ATG5 — ATG5 Protein63%
related📖LC3 (MAP1LC3) Neurons63%
related📖MAP1LC3A Gene63%
related📖LC3 Protein63%
related📖LC3 (Microtubule-Associated Protein 1 Light Chain 3)63%
related📖P2RY12 Gene63%
related📖SQSTM1/p6263%
related📖SQSTM1 — Sequestosome 1 (p62)63%
related📖SQSTM1 Protein (p62/Sequestosome-1)63%
related📖SQSTM1/p62 Protein63%
mentions📖Autophagy60%
mentions📖researchers60%
mentions📖Vascular Smooth Muscle Cells60%
mentions📖autophagy60%
related📖autophagy55%
related📖Autophagy54%
related📖researchers54%
related📖Vascular Smooth Muscle Cells54%
related📖autophagy54%
mentions📖Macroautophagy Dysfunction in PD - Experiment Design50%
mentions📖Macroautophagy Dysfunction Validation Framework in Parkinson50%
mentions📖Autophagy-Lysosomal Dysfunction Neurons50%
mentions📖Autophagy-Lysosomal Impairment Comparison — AD/PD/ALS/FTD/HD50%
mentions📖Autophagy-Lysosomal Dysfunction in Neurodegenerative Disease50%
mentions📖autophagy-lysosome-dysfunction50%
mentions📖Autophagy-Lysosome Pathway in Neurodegeneration50%
mentions📖mHTT Clearance Mechanisms in Huntington's Disease50%
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mentions📖Autophagy Types in Neurodegeneration50%
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mentions📖Cerebellar Interneurons in Neurodegeneration50%
mentions📖Medial Forebrain Bundle in Neurodegeneration50%
mentions📖Ventral Tegmental Area Dopamine Neurons in Neurodegeneration50%
mentions📖Microglia CD8+ T Cell Recruitment -- Supplementary WW Conten50%
mentions📖TREM2 Mechanism Hub50%
mentions📖P2Y12 Receptor Antagonists for Neurodegeneration50%
mentions📖Microglia50%
mentions📖Trained Innate Immunity Reset Therapy for Neurodegeneration50%
mentions📖neuroinflammation50%
mentions📖P2Y12 Receptor Antagonist Therapy50%
mentions📖iPSC-Derived Microglia50%
mentions📖Microglia State Index50%
mentions📖Microglial Cells — Cell Type Hierarchy50%
mentions📖P2Y12 Neurons50%
mentions📖Microglia Activation Mechanism50%
mentions📖Autophagic Cell Death Pathway in Neurodegeneration44%
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mentions📖STING-CASM-GABARAP-LRRK2 Pathway in Parkinson's Disease44%
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mentions📖Microglia in Chronic Neuroinflammation44%
mentions📖Microglia in Neuroinflammation44%
mentions📖Microglial Aging and Immune Memory in Neurodegeneration — Tr44%
mentions📖CBS Single-Cell Transcriptomics Mechanisms44%
mentions📖Microglia Dysfunction: AD vs PD vs ALS vs FTD vs HD Comparis44%
mentions📖Microglia and neuroinflammation in Alzheimer's Disease44%
mentions📖Microglial State Trajectory from Mobilization to Dysregulati44%
mentions📖Purinergic Signaling in Neurodegeneration44%
mentions📖Copper and Zinc Homeostasis in CBS/PSP44%
mentions📖Section 180: Copper and Zinc Homeostasis in CBS/PSP44%