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Fig. 5: SIRT1 knockdown abolishes the protection of PDE4D silencing on ISO-induced hyper...
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Fig. 5Figure 6
SIRT1 knockdown abolishes the protection of PDE4D silencing on ISO-induced hypertrophy and mitochondrial dysfunction in cardiomyocytes. A through F, NRVMs were transfected with PDE4D siRNA (siPDE4D) and SIRT1 shRNA or negative control siRNA (siNC) for 48 h followed by vehicle or ISO (10 μM) treatment for another 24 h. A, Immunofluorescence staining of α-actinin (red) and DAPI (blue) and quantification of myocytes area in NRVMs; n = 24 cells from 3 independent experiments. Scale bar, 20 μm. B, FACS analyses of ΔΨm in NRVMs. A loss of ΔΨm was demonstrated by the ratio of JC-1 aggregates to JC-1 monomers; n = 3 independent experiments. C, DCFH-DA staining and quantification of cellular ROS levels in NRVMs; n = 8 independent experiments. Scale bar, 50 μm. D, MitoSOX staining and quantification of mitochondrial ROS levels in NRVMs; n = 8 independent experiments. Scale bar, 10 μm. E , Representative immunofluorescence images of SIRT1 staining (red) and DAPI staining (blue) in NRV
▸Metadata
| doi | 10.1016/j.redox.2025.103563 |
| pmcid | PMC11909752 |
| _origin | {'url': 'https://www.ebi.ac.uk/europepmc/articles/PMC11909752/bin/gr5.jpg', 'type': 'external', 'tracked_at': '2026-04-23T01:08:31.372313'} |
| caption | SIRT1 knockdown abolishes the protection of PDE4D silencing on ISO-induced hypertrophy and mitochondrial dysfunction in cardiomyocytes. A through F, NRVMs were transfected with PDE4D siRNA (siPDE4D |
| paper_id | 9a10f9ee-2201-47b2-a4ca-b169666a53d9 |
| image_url | https://www.ebi.ac.uk/europepmc/articles/PMC11909752/bin/gr5.jpg |
| image_path | |
| description | |
| figure_label | Fig. 5 |
| figure_number | 6 |
| _schema_version | 1 |
| source_strategy | pmc_api |
| entities_mentioned |
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