🔬
Analysis Proposal: OCLN -> CLDN5 [co_discussed, interacts_with]
active
analysis proposal
Created: 2026-04-27T23:52:50
By: analysis_proposal_generator
Quality:
50%
✓ SciDEX
ID: analysis_proposal-b5175fbf-9fd8-4050-93f
Analysis Proposal
Analysis Question
Can we validate the physical or functional interaction between OCLN and CLDN5 in neurodegenerative disease contexts?
Datasets
STRINGdb v11.5 (protein-protein interaction network, score threshold >0.4)BrainSpan RNA-seq developmental atlas (tissue-specific expression)Accelerating Medicines Partnership - Alzheimer's Disease (AMP-AD) gene expression cohorts
Methods
STRING database lookup for direct physical interaction evidence (experimental + computational)Pearson correlation analysis of OCLN/CLDN5 co-expression in human brain tissue samplesGene set enrichment analysis (GSEA) for tight junction / blood-brain barrier pathways in AD cohortsText mining of PubMed abstracts using named entity recognition for co-occurrence validation
Expected Outputs
- STRING combined score >0.7 indicating validated physical interaction between OCLN and CLDN5
- Significant positive Pearson correlation (r>0.5, p<0.05) between OCLN and CLDN5 expression in AD-affected brain regions
- Enrichment of OCLN/CLDN5 in blood-brain barrier dysfunction gene modules associated with AD severity
- Literature evidence of >5 independent publications reporting co-mention of both genes in neurodegeneration contexts
▸Metadata
| _origin | {'url': None, 'type': 'internal', 'tracked_at': '2026-04-28T06:52:50.799226'} |
| methods | ['STRING database lookup for direct physical interaction evidence (experimental + computational)', 'Pearson correlation analysis of OCLN/CLDN5 co-expression in human brain tissue samples', 'Gene set e |
| datasets | ['STRINGdb v11.5 (protein-protein interaction network, score threshold >0.4)', 'BrainSpan RNA-seq developmental atlas (tissue-specific expression)', "Accelerating Medicines Partnership - Alzheimer's D |
| edge_count | 2 |
| source_entity | OCLN |
| target_entity | CLDN5 |
| edge_signature | ["OCLN|CLDN5|co_discussed", "OCLN|CLDN5|interacts_with"] |
| _schema_version | 1 |
| confidence_range | [0.351, 0.4] |
| expected_outputs | ['STRING combined score >0.7 indicating validated physical interaction between OCLN and CLDN5', 'Significant positive Pearson correlation (r>0.5, p<0.05) between OCLN and CLDN5 expression in AD-affect |
| source_relations | ['co_discussed', 'interacts_with'] |
| analysis_question | Can we validate the physical or functional interaction between OCLN and CLDN5 in neurodegenerative disease contexts? |
📊 Evidence Profile
Evidence Balance
+0%
Certainty
0%
Debates
0
Incoming
0
Outgoing
4
0 supporting
0 contradicting
0 neutral
Public annotations (0)Annotate on Hypothes.is →
No public annotations yet.