🧫
Trehalose-induced autophagy gene expression analysis
active
experiment
Created: 2026-04-06T12:29:04
By: etl-v1-backfill
Quality:
50%
✓ SciDEX
ID: exp-a46aae09-e01f-4e92-a9d0-99a2f1697938
🧫 Experiment Protocol
Exploratorymotoneuron degenerationmultiple autophagy genesNSC34 neuroblastoma x spinal cord cellsproposed
This experiment examined the transcriptional changes induced by trehalose treatment in cellular models. Using RT-qPCR, the researchers measured expression levels of TFEB target genes and autophagy-related genes including Ppargc1a, lysosomal hydrolases (Ctsb, Gla, Tpp1), lysosomal membrane proteins (Lamp2a, Mcoln1), and autophagy components (Becn1, Atg10, Atg12, Sqstm1/p62, Map1lc3b, Hspb8, Bag3). The study demonstrated that most of these changes were dependent on PPP3 and TFEB activity.
PRIMARY OUTCOME
mRNA expression levels of autophagy and lysosomal genes
EXPECTED OUTCOMES
Upregulation of TFEB target genes and autophagy-related genes in a PPP3- and TFEB-dependent manner
SUCCESS CRITERIA
Significant upregulation of target genes that is blocked by PPP3 inhibition or TFEB silencing
PROTOCOL
RT-qPCR analysis of gene expression following trehalose treatment, with and without PPP3 inhibition or TFEB silencing
LINKED HYPOTHESES
Source: PMID 30335591 ↗
🧫 Experiment Extras
PATHWAY
autophagy/lysosome pathway
MARKET PRICE
$0.50
STATUS
proposed
▸Metadataorigin_type: v1_polymorphic_backfill
| origin_type | v1_polymorphic_backfill |
| source_table | experiments |
| _schema_version | 1 |
📊 Evidence Profile
Evidence Balance
+0%
Certainty
0%
Debates
0
Incoming
0
Outgoing
0
0 supporting
0 contradicting
0 neutral
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