Trehalose-induced autophagy gene expression analysis

Exploratory Score: 0.850 Price: $0.50 motoneuron degeneration NSC34 neuroblastoma x spinal cord cells Status: proposed

What This Experiment Tests

Exploratory experiment designed to discover new patterns targeting multiple autophagy genes in NSC34 neuroblastoma x spinal cord cells. Primary outcome: mRNA expression levels of autophagy and lysosomal genes

Description

This experiment examined the transcriptional changes induced by trehalose treatment in cellular models. Using RT-qPCR, the researchers measured expression levels of TFEB target genes and autophagy-related genes including Ppargc1a, lysosomal hydrolases (Ctsb, Gla, Tpp1), lysosomal membrane proteins (Lamp2a, Mcoln1), and autophagy components (Becn1, Atg10, Atg12, Sqstm1/p62, Map1lc3b, Hspb8, Bag3). The study demonstrated that most of these changes were dependent on PPP3 and TFEB activity.

TARGET GENE

multiple autophagy genes

MODEL SYSTEM

NSC34 neuroblastoma x spinal cord cells

ESTIMATED COST

TIMELINE

0 months

PATHWAY

autophagy/lysosome pathway

SOURCE

extracted_from_pmid_30335591

PRIMARY OUTCOME

mRNA expression levels of autophagy and lysosomal genes

Scoring Dimensions

📖 Wiki Pages

tfeb-activators-neurodegenerationtherapeutic TFEB Activators in Neurodegenerationtherapeutic TFEB Activator Therapies for Neurodegenerative Distherapeutic TFEB Proteinprotein TFEB Protein (Transcription Factor EB)protein TFEB Signaling in Neurodegenerationmechanism TFEB Autophagy Pathwaymechanism TFEB Activators for Parkinson's Diseasemechanism TFEB-Mediated Lysosomal Biogenesismechanism TFEBgene TFEB (Redirect)redirect Researchersindex LAMP2A Protein — Lysosomal-Associated Membrane Proprotein autophagymechanism LAMP2A Genegene

Protocol

RT-qPCR analysis of gene expression following trehalose treatment, with and without PPP3 inhibition or TFEB silencing

Expected Outcomes

Upregulation of TFEB target genes and autophagy-related genes in a PPP3- and TFEB-dependent manner

Success Criteria

Significant upregulation of target genes that is blocked by PPP3 inhibition or TFEB silencing

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