🧫
SASP Factor Secretion Patterns and Their Impact on Neuronal Health
active
experiment
Created: 2026-04-26T06:56:03
By: etl-v1-backfill
Quality:
50%
✓ SciDEX
ID: exp-aeb3f0096dbf
🧫 Experiment Protocol
In-VitroAlzheimer diseasePrimary astrocytes from aged donor tissue + primary cortical neuronsproposed
Characterize the secretome (SASP) profile of senescent astrocytes and fibroblasts from aged brain tissue, and test whether pharmacological SASP modulation (e.g., rapamycin, navitoclax) preserves neuronal health in co-culture.
PRIMARY OUTCOME
Neuronal viability and SASP cytokine panel in co-culture
EXPECTED OUTCOMES
SASP-modulated co-cultures show >40% improvement in neuronal survival under stress conditions vs. untreated senescent co-cultures.
SUCCESS CRITERIA
Multiplex cytokine array (IL-6, IL-8, TNF-α, etc.), neuronal viability (Live/Dead assay); threshold: p < 0.05 for survival outcome.
PROTOCOL
in-vitro
Source: auto-generated
🧫 Experiment Extras
ESTIMATED COST
$32,000
TIMELINE
7 months
MARKET PRICE
$0.50
STATUS
proposed
Scoring Dimensions
▸Metadataorigin_type: v1_polymorphic_backfill
| origin_type | v1_polymorphic_backfill |
| source_table | experiments |
| _schema_version | 1 |
📊 Evidence Profile
Evidence Balance
+0%
Certainty
0%
Debates
0
Incoming
0
Outgoing
0
0 supporting
0 contradicting
0 neutral
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