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FIGURE 5 — Connexin43 Deficiency Leads to Ventricular Arrhythmias by Reprogramming Proline

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paper figure Created: 2026-04-21T18:29:40 By: paper_figures_tool Quality: 50% 🔗 External ID: paper-fig-paper-07c5d7883c4f-5
FIGURE 5 — Connexin43 Deficiency Leads to Ventricular Arrhythmias by Reprogramming Proline
FIGURE 5Figure 5
ROS‐mediated abnormal calcium handling confers the arrhythmic phenotype of Cx43‐KO iPSC‐CMs. (A) Representative calcium (Ca 2+ ) transient waveforms recorded by ratiometric Fura‐2 imaging from WT and Cx43‐KO iPSC‐CMs. (B–H) Bar graphs to compare key parameters of calcium transients between WT and KO iPSC‐CMs, including diastolic calcium, calcium amplitude, peak calcium, maximal rising rate, maximal decay rate, time to peak, and transient duration 90. n = 40 cells. (I) Mitochondrial calcium analysis through Rhod‐2 staining in WT and Cx43‐KO iPSC‐CMs with or without ISO (100 n m , 2 h) stimulation, and with or without treatment of either Mito‐TEMPO (MT, 5 µ m , 72 h) or N ‐acetyl‐ l ‐cysteine (NAC, 5 m m , 4 h). n = 4–6 independently biological repeats. (J) Representative action potential waveforms recorded by single‐cell patch clamp from Cx43‐KO iPSC‐CMs treated with phosphate‐buffered saline (PBS, vehicle), NAC (5 m m , 4 h) or proline (Pro, 100 n m , 24 h). Dash lines indicate 0
PubMed: paper-07c5d7883c4f
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pmidpaper-07c5d7883c4f
captionROS‐mediated abnormal calcium handling confers the arrhythmic phenotype of Cx43‐KO iPSC‐CMs. (A) Representative calcium (Ca 2+ ) transient waveforms recorded by ratiometric Fura‐2 imaging from WT and
image_urlhttps://www.ebi.ac.uk/europepmc/articles/PMC13045320/bin/ADVS-13-e16090-g004.jpg
paper_titleConnexin43 Deficiency Leads to Ventricular Arrhythmias by Reprogramming Proline Metabolism.
figure_labelFIGURE 5
figure_number5
_schema_version1
source_strategypmc_api
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