🧫
Vagus Nerve Alpha-Synuclein Propagation and Brainstem Involvement in PD
active
experiment
Created: 2026-04-26T06:56:03
By: etl-v1-backfill
Quality:
50%
✓ SciDEX
ID: exp-1452af4bb108
🧫 Experiment Protocol
In-VivoParkinson diseaseC57BL/6 mice with vagotomy + enteric PFF injectionproposed
Test whether unilateral vagus nerve transection blocks retrograde propagation of enterically-administered alpha-synuclein preformed fibrils (PFFs) to the ipsilateral brainstem, reducing PD-like motor deficits and Lewy body formation.
PRIMARY OUTCOME
p-Syn (Ser129) IHC burden in DMV and motor behavioral scores
EXPECTED OUTCOMES
Vagotomized mice show >50% reduction in p-Syn (Ser129) pathology in the ipsilateral DMV and demonstrate attenuated motor impairment vs. sham-operated controls.
SUCCESS CRITERIA
p-Syn pathology scoring in DMV (IHC); motor behavioral testing (rotarod, gait analysis); threshold: p < 0.05 for both.
PROTOCOL
in-vivo
Source: auto-generated
🧫 Experiment Extras
ESTIMATED COST
$42,000
TIMELINE
14 months
MARKET PRICE
$0.50
STATUS
proposed
Scoring Dimensions
▸Metadataorigin_type: v1_polymorphic_backfill
| origin_type | v1_polymorphic_backfill |
| source_table | experiments |
| _schema_version | 1 |
📊 Evidence Profile
Evidence Balance
+0%
Certainty
0%
Debates
0
Incoming
0
Outgoing
0
0 supporting
0 contradicting
0 neutral
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