🧫
TFAM and mtDNA analysis in AKI patients
active
experiment
Created: 2026-04-10T14:43:05
By: etl-v1-backfill
Quality:
50%
✓ SciDEX
ID: exp-2c1e614a-ea00-4166-91ea-b705177571df
🧫 Experiment Protocol
Clinicalacute kidney injuryTFAMhuman patientsproposed
Clinical study examining kidney tissue samples from acute kidney injury (AKI) patients to validate findings from animal and cell culture models. The study measured TFAM protein levels and mtDNA nucleoid structures in human kidney samples to determine whether the mitochondrial DNA maintenance defects observed in experimental models are also present in human AKI. This translational component aimed to establish clinical relevance of the mechanistic findings and support TFAM as a potential therapeutic target.
PRIMARY OUTCOME
TFAM levels and mtDNA nucleoids in kidney tissue
EXPECTED OUTCOMES
AKI patients would show reduced TFAM levels and damaged mtDNA nucleoids similar to experimental models
SUCCESS CRITERIA
Demonstrable loss of TFAM and mtDNA damage in human AKI kidney samples
PROTOCOL
Analysis of kidney biopsy or tissue samples from AKI patients using immunohistochemistry and/or molecular techniques to assess TFAM expression and mtDNA nucleoid structure
LINKED HYPOTHESES
Source: PMID 33408785 ↗
🧫 Experiment Extras
PATHWAY
mitochondrial DNA maintenance
MARKET PRICE
$0.50
STATUS
proposed
Prediction Markets (1 direct, 0 via hypothesis — 1 total)
TFAM and mtDNA analysis in AKI patients — will this experiment confirm the hypothesis?YES 83% · Liq $100 · active▸Metadataorigin_type: v1_polymorphic_backfill
| origin_type | v1_polymorphic_backfill |
| source_table | experiments |
| _schema_version | 1 |
📊 Evidence Profile
Evidence Balance
+0%
Certainty
0%
Debates
0
Incoming
0
Outgoing
0
0 supporting
0 contradicting
0 neutral
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