TFAM

Transcription factor A, mitochondrial

Score: 0.576 Price: $0.58 Medium Druggability Status: active Wiki: TFAM
๐Ÿง  Neurodegeneration
HYPOTHESES
9
PAPERS
28
KG EDGES
451
DEBATES
0

3D Protein Structure

🧬 TFAM โ€” PDB 3TMM Click to expand

Experimental structure from RCSB PDB | Powered by Mol*

Druggability & Clinical Context

Druggability
Medium
Score: 0.50
Clinical Stage
Approved
Target Class
Transcription Factor
Safety
0.40
Druggability Analysis
Drug Development0.80
Structural Tractability0.85
Target Class0.50
Safety Profile0.40
Key Metrics
PDB Structures:
16
Known Drugs:
4
Approved:
1
In Clinical Trials:
1
Drug Pipeline (4 compounds)
1 Approved ยท 1 Preclinical
Druggability Rationale: TFAM presents medium druggability (0.55 score) due to its transcription factor nature, which traditionally poses challenges for small-molecule inhibition; however, the target benefits from well-characterized structural data (16 PDB structures at 1.35 ร… resolution), established indirect activation pathways via PGC-1ฮฑ/SIRT1 modulators, and clinical precedent with mitochondrial-targeted therapeutics (MitoQ in Phase 2), making indirect/allosteric approaches more viable than direct DNA-binding site targeting.
Mechanism: Drugs targeting TFAM would enhance or modulate its transcriptional activity to increase mitochondrial DNA replication and gene expression, thereby boosting mitochondrial biogenesis and ATP production. Alternatively, indirect approaches activate upstream regulators like PGC-1ฮฑ and SIRT1 to increase TFAM expression and activity.
Drug Pipeline (4 compounds)
1 Approved ยท 1 Preclinical
Known Drugs:
Bezlotoxumab (approved) โ€” Clostridium difficile infection recurrence prevention
Urolithin A (preclinical) โ€” Mitochondrial dysfunction and age-related muscle weakness
PGC-1ฮฑ activators (SIRT1 modulators) (research) โ€” Mitochondrial biogenesis enhancement in metabolic diseases
Mitochondrial-targeted antioxidants (MitoQ) (phase2) โ€” Neurodegenerative diseases and mitochondrial dysfunction
Structural Data:
PDB (16) โœ“AlphaFold โœ“Cryo-EM โœ“
3FGH3TMM3TQ64NNU4NOD+11 more
UniProt: Q6LES8

🧬 3D Protein Structure

🧬 TFAM — PDB 3TMM Click to expand interactive 3D viewer

Experimental structure from RCSB PDB | Powered by Mol* | Rotate: click+drag | Zoom: scroll

Selectivity & Safety Considerations

TFAM selectivity is primarily a challenge in distinguishing mitochondrial transcription modulation from nuclear transcription factor off-targets; mitochondrial-targeted delivery approaches (e.g., MitoQ strategy) provide spatial selectivity, while indirect activation through SIRT1/PGC-1ฮฑ pathways may have broader metabolic effects requiring careful dose titration to minimize systemic side effects.

3D Protein Structure

PDB: Open in RCSB AlphaFold model

Interactive 3D viewer powered by RCSB PDB / Mol*. Use mouse to rotate, scroll to zoom.

Clinical Trials (8)

Relevant trials from ClinicalTrials.gov

Active
3
Completed
5
Total Enrollment
1,067
By Phase
NA: 4 ยท PHASE2: 2 ยท PHASE3: 1 ยท PHASE4: 1
Placebo-Controlled Trial of Urolithin A Supplementation in Men With Prostate Cancer Undergoing Radical Prostatectomy, UR Recruiting
PHASE2 NCT06022822 n=90
Prostate Adenocarcinoma
Interventions: Biopsy Procedure, Biospecimen Collection, Placebo Administration
Sponsor: National Cancer Institute (NCI) | Started: 2024-09-12
Urolithin A in Patients With Previously Untreated Solid Tumors Receiving Immune Checkpoint Inhibitors Recruiting
NA NCT07161310 n=45
Solid Cancer, Non-Small Cell Lung Cancer, Melanoma
Interventions: Urolithin A, Placebo, Bio specimens
Sponsor: Goethe University | Started: 2026-01-02
Impact of a Novel Brain Longevity Supplement Containing the Postbiotic Urolithin A (Mitopure) on Cognitive Function and Active Not Recruiting
NA NCT07060898 n=650
Aging, Cognitive Decline, Brain Fog
Interventions: Active Product, Placebo Product
Sponsor: Amazentis SA | Started: 2025-07-29
Bezlotoxumab (BEZLO) In Addition To Standard Of Care (SOC) Vancomycin For The Treatment of Multi-Recurrent Clostridium D Completed
PHASE4 NCT03880539 n=4
Clostridium Difficile Infection Recurren
Interventions: bezlotoxumab, Vancomycin Oral
Sponsor: University of Kansas Medical Center | Started: 2019-06-25
Bezlotoxumab (MK-6072) Versus Placebo in Children With Clostridium Difficile Infection (CDI) (MK-6072-001) Completed
PHASE3 NCT03182907 n=148
Clostridium Difficile Infection
Interventions: Bezlotoxumab, Placebo, Antibacterial drug treatment (ABD)
Sponsor: Merck Sharp & Dohme LLC | Started: 2018-03-27
Bezlotoxumab Yielded Outcomes by Addressing Personalized Needs in Clostridioides Difficile Infection Completed
PHASE2 NCT05304715 n=44
Clostridioides Difficile Infection, Stool Microbiome, Organ Dysfunction Syndrome
Interventions: Bezlotoxumab, Normal saline 0.9% or 5% dextrose water
Sponsor: Hellenic Institute for the Study of Sepsis | Started: 2022-04-13
Evaluation of the Anti-aging Efficacy of One Face Care Cosmetic Product Completed
NA NCT06619457 n=36
Skin Aging
Interventions: Mitopure (Urolithin A) Cream, Placebo Cream
Sponsor: Amazentis SA | Started: 2024-09-25
Urolithin A Supplementation in Middle-aged Adults With Obesity Completed
NA NCT05921266 n=50
Obesity, Vascular Dementia, Cognitive Impairment
Interventions: Urolithin A, Placebo
Sponsor: University of Oklahoma | Started: 2023-10-02

Linked Hypotheses (1)

TFAM overexpression creates mitochondrial donor-recipient gradients for directed organelle trafficki0.725

Linked Experiments (5)

IRI-AKI mouse model with mtROS inhibition0.900
mtROS effects on TFAM and mtDNA in HK2 cells0.900
TFAM knockdown functional analysis0.850
TFAM and mtDNA analysis in AKI patients0.800
Proposed experiment from debate on Mitochondrial transfer between astrocytes and neurons0.400

Scoring Dimensions

Portfolio 0.61 (25%) Druggability 0.50 (20%) Evidence 0.66 (20%) Safety 0.40 (15%) Competitive 0.40 (10%) Connectivity 0.90 (10%) 0.576 composite

Knowledge Graph (20)

activates (3)

NRF1 โ†’ TFAM
NRF2 โ†’ TFAM
TFAM โ†’ MITOCHONDRIAL_DNA

associated with (11)

TFAM โ†’ neurodegeneration
MAP1LC3B โ†’ TFAM
GPX4 โ†’ TFAM
BMAL1 โ†’ TFAM
LC3 โ†’ TFAM
...and 6 more

co discussed (6)

COX4I1 โ†’ TFAM
PRKAA1 โ†’ TFAM
GJA1 โ†’ TFAM
RAB27A โ†’ TFAM
GAP43 โ†’ TFAM
...and 1 more

Debate History (0)

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