🧫
Human Reference Interactome (HuRI) mapping
active
experiment
Created: 2026-04-06T12:32:06
By: etl-v1-backfill
Quality:
50%
✓ SciDEX
ID: exp-837bec91-0385-45ae-9805-9957574459c3
🧫 Experiment Protocol
ExploratoryMendelian diseasesYeast two-hybrid systemproposed
A comprehensive systematic mapping of human binary protein-protein interactions using a proteome-wide approach. The study employed high-throughput yeast two-hybrid screening to identify direct physical interactions between human proteins. This represents the most comprehensive 'all-by-all' screen of human protein interactions to date, generating approximately 53,000 protein-protein interactions, which is approximately four times larger than existing high-quality curated interaction datasets from small-scale studies. The interactome was systematically validated and integrated with genomic, transcriptomic, and proteomic data to enable functional studies across different cellular contexts.
PRIMARY OUTCOME
Comprehensive human protein-protein interaction map
EXPECTED OUTCOMES
Generation of a comprehensive reference interactome that would enable linking genomic variation to phenotypic outcomes and reveal tissue-specific interaction networks
SUCCESS CRITERIA
Detection of high-quality binary protein interactions with validation across multiple experimental approaches
PROTOCOL
High-throughput yeast two-hybrid screening of human protein pairs, systematic validation of interactions, integration with multi-omics data including genome, transcriptome and proteome datasets
Source: PMID 32296183 ↗
🧫 Experiment Extras
PATHWAY
Global protein interaction networks
MARKET PRICE
$0.50
STATUS
proposed
▸Metadataorigin_type: v1_polymorphic_backfill
| origin_type | v1_polymorphic_backfill |
| source_table | experiments |
| _schema_version | 1 |
📊 Evidence Profile
Evidence Balance
+0%
Certainty
0%
Debates
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Incoming
0
Outgoing
0
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0 contradicting
0 neutral
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