Exploratory experiment designed to discover new patterns targeting C1QA, C1QC, SPI1 in RAW264.7 macrophages. Primary outcome: Confirmation of high expression levels of C1QA, C1QC, and SPI1
RAW264.7 macrophages were treated with oxidized low-density lipoprotein (ox-LDL) to simulate atherosclerotic conditions. Quantitative real-time PCR (qRT-PCR) was performed to confirm the expression levels of hub genes C1QA, C1QC, and the transcription factor SPI1. This experiment validated the computational findings from the transcriptomic analysis by demonstrating that these genes are indeed upregulated in macrophages exposed to ox-LDL, which is a key trigger of atherosclerotic inflammation.
ox-LDL treatment of RAW264.7 macrophages followed by qRT-PCR analysis
Upregulation of C1QA, C1QC, and SPI1 expression
Statistically significant increase in gene expression levels
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