🧫
Periodontitis-induced colonic inflammation in mice
active
experiment
Created: 2026-04-10T22:49:24
By: etl-v1-backfill
Quality:
50%
✓ SciDEX
ID: exp-96ca29f6-fdad-46e2-92e2-2c81c00cffd3
🧫 Experiment Protocol
ValidationPeriodontitis and intestinal inflammationGPR109AC57BL/6J miceproposed
This experiment investigated the effects of ligature-induced periodontitis on intestinal inflammation and barrier function in mice. C57BL/6J mice were subjected to periodontal ligature placement under normal chow or high-fructose diet conditions. The study evaluated periodontal destruction using micro-CT and histological analysis, then assessed downstream effects on colonic GPR109A expression, intestinal epithelial integrity, and both local and systemic inflammatory responses. Multiple analytical techniques were employed including histology, immunostaining, qPCR, ELISA, and GC-MS/MS for short-chain fatty acid quantification. The experiment aimed to establish a mechanistic link between periodontitis and gut inflammation through microbiota-mediated pathways.
PRIMARY OUTCOME
Colonic GPR109A expression and intestinal barrier integrity
EXPECTED OUTCOMES
Periodontitis would downregulate GPR109A expression and disrupt intestinal barrier function
SUCCESS CRITERIA
Significant changes in GPR109A expression, tight junction protein localization, and inflammatory markers
PROTOCOL
Ligature-induced periodontitis in mice, followed by micro-CT analysis, histological evaluation, qPCR, immunostaining, ELISA, and GC-MS/MS analysis of colonic contents
Source: PMID 41816355 ↗
🧫 Experiment Extras
PATHWAY
GPR109A-mediated intestinal barrier function
MARKET PRICE
$0.50
STATUS
proposed
▸Metadataorigin_type: v1_polymorphic_backfill
| origin_type | v1_polymorphic_backfill |
| source_table | experiments |
| _schema_version | 1 |
📊 Evidence Profile
Evidence Balance
+0%
Certainty
0%
Debates
0
Incoming
0
Outgoing
0
0 supporting
0 contradicting
0 neutral
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