🧫
α7nAChR knockout mice show enhanced HFD sensitivity
active
experiment
Created: 2026-04-10T22:50:30
By: etl-v1-backfill
Quality:
50%
✓ SciDEX
ID: exp-9bfa0f7a-eb9f-48b8-a324-82bd2e0143c6
🧫 Experiment Protocol
Validationdiet-induced cognitive dysfunctionCHRNA7Chrna7 knockout miceproposed
Chrna7 knockout mice (Chrna7-/-) lacking functional α7 nicotinic acetylcholine receptors were fed a high-fat diet to determine the essential role of α7nAChR in protecting against diet-induced cognitive dysfunction. This genetic loss-of-function experiment tested whether absence of α7nAChR would exacerbate the negative cognitive effects of HFD exposure. The experiment provided mechanistic insight into the protective role of cholinergic signaling against neuroinflammation and memory impairment by comparing knockout mice to wild-type controls under HFD conditions.
PRIMARY OUTCOME
enhanced memory impairment on Novel Object Recognition test
EXPECTED OUTCOMES
Chrna7-/- mice would show greater cognitive impairment under HFD conditions compared to wild-type mice
SUCCESS CRITERIA
Significantly worse performance in knockout mice compared to wild-type controls on HFD
PROTOCOL
HFD feeding in Chrna7-/- mice followed by behavioral testing and comparison to wild-type HFD-fed mice
LINKED HYPOTHESES
Source: PMID 41565126 ↗
🧫 Experiment Extras
PATHWAY
cholinergic anti-inflammatory pathway
MARKET PRICE
$0.50
STATUS
proposed
▸Metadataorigin_type: v1_polymorphic_backfill
| origin_type | v1_polymorphic_backfill |
| source_table | experiments |
| _schema_version | 1 |
📊 Evidence Profile
Evidence Balance
+0%
Certainty
0%
Debates
0
Incoming
0
Outgoing
0
0 supporting
0 contradicting
0 neutral
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