MAP6 depletion effects on neuronal development in primary cultures

Exploratory Score: 0.900 Price: $0.50 primary rodent neuronal cultures Status: proposed

What This Experiment Tests

Exploratory experiment designed to discover new patterns targeting MAP6 in primary rodent neuronal cultures. Primary outcome: rate of neuronal development, growth cone turning efficiency, neuronal process number, axonal branch

Description

This experiment investigated the effects of MAP6 protein depletion on neuronal development parameters using primary rodent neuronal cultures. MAP6 association with microtubules causes them to be stable, opposite to tau's effect. The researchers examined how MAP6 depletion affects the rate of neuronal development, growth cone turning efficiency, and the number of neuronal processes and axonal branches. The study was designed to compare MAP6's effects with those of tau depletion to understand their antagonistic relationship in regulating neuronal morphology through microtubule stability modulation.

TARGET GENE
MODEL SYSTEM
primary rodent neuronal cultures
ESTIMATED COST
$0
TIMELINE
0 months
PATHWAY
microtubule dynamics
SOURCE
extracted_from_pmid_39257379
PRIMARY OUTCOME
rate of neuronal development, growth cone turning efficiency, neuronal process number, axonal branch number

Scoring Dimensions

Info Gain 0.00 (25%) Feasibility 0.00 (20%) Hyp Coverage 0.00 (20%) Cost Effect. 0.00 (15%) Novelty 0.00 (10%) Ethical Safety 0.00 (10%) 0.900 composite

📖 Wiki Pages

MAP6 GenegeneMAP6 Protein — Microtubule-Associated Protein 6proteinResearchersindexviral-post-infectious-alsmechanismTau Immunotherapy MechanismsmechanismNeuronal MigrationmechanismNeuronal Cytoskeleton Dynamics in 4R-TauopathiesmechanismEpigenetics in Amyotrophic Lateral SclerosismechanismSleep and Respiratory Network Interaction in ALS —experimentMicrobiome-Gut Barrier Signatures in ALS — ExperimexperimentEnvironmental Exposure Causal Attribution in ALS —experimentAmyotrophic Lateral Sclerosis (ALS)diseaseHigh-Dose Vitamins ALS TrialclinicalSodium Phenylbutyrate ALS TrialclinicalSargramostim (GM-CSF) ALS Trialclinical

Protocol

MAP6 depletion in primary neuronal cultures with measurement of developmental parameters

Expected Outcomes

opposite effects to tau depletion on neuronal development parameters

Success Criteria

measurable changes opposite to those seen with tau depletion

Related Hypotheses (1)

Tau-Independent Microtubule Stabilization via MAP6 Enhancement0.480

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