TLR4-NF-κB-NLRP3 Axis Inhibition in iPSC-derived Microglia for AD Prevention

In-Vitro Score: 0.850 Price: $0.50 Alzheimer disease iPSC-derived microglia-neuron co-culture from AD patients Status: proposed

What This Experiment Tests

In-Vitro experiment designed to assess clinical efficacy targeting N/A in iPSC-derived microglia-neuron co-culture from AD patients. Primary outcome: NLRP3 inflammasome activation markers (IL-1β, IL-18) and neuronal viability (TUNEL/cleaved caspase-3

Description

Evaluate whether selective TLR4-NF-κB-NLRP3 axis inhibition reduces neuroinflammatory burden and prevents neuronal loss in Alzheimer disease iPSC-derived microglia-neuron co-culture models.

TARGET GENE

N/A

MODEL SYSTEM

iPSC-derived microglia-neuron co-culture from AD patients

ESTIMATED COST

$45,000

TIMELINE

8 months

PATHWAY

N/A

SOURCE

auto-generated

PRIMARY OUTCOME

NLRP3 inflammasome activation markers (IL-1β, IL-18) and neuronal viability (TUNEL/cleaved caspase-3)

Scoring Dimensions

Protocol

in-vitro

Expected Outcomes

Reduction in NLRP3 inflammasome activation (IL-1β, IL-18 release) by >50% and decreased neuronal apoptosis in co-culture.

Success Criteria

NLRP3 activation assay (ELISA) + TUNEL staining neuronal viability; threshold: p < 0.05 vs. LPS-stimulated control.

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