🧫
NAD+ Boosting and SIRT1 Activation to Reverse Cellular Senescence in Neurons
active
experiment
Created: 2026-04-26T06:56:03
By: etl-v1-backfill
Quality:
50%
✓ SciDEX
ID: exp-43fa40a6043e
🧫 Experiment Protocol
In-VitroAlzheimer diseasePrimary cortical neurons from aged (18-month) mice or SAMP8completed
Evaluate whether NAD+ precursor supplementation (NMN) activates SIRT1/PGC1α signaling, restores mitochondrial function, and reduces senescence markers in aged neurons and senescence-accelerated mouse model (SAMP8).
PRIMARY OUTCOME
NAD+/NADH ratio, SA-β-gal positivity, mitochondrial OCR
EXPECTED OUTCOMES
NMN treatment increases NAD+/NADH ratio >2-fold, elevates SIRT1 activity, reduces SA-β-gal positivity by >40%, and restores mitochondrial oxygen consumption rate (OCR).
SUCCESS CRITERIA
NAD+/NADH ratio (colorimetric assay), SA-β-gal assay, mitochondrial OCR (Seahawk); threshold: p < 0.05 vs. aged vehicle control.
PROTOCOL
in-vitro
Source: auto-generated
🧫 Experiment Extras
ESTIMATED COST
$35,000
TIMELINE
8 months
MARKET PRICE
$0.50
STATUS
completed
Scoring Dimensions
Experiment Results (1)
PARTIALConfidence: 68%
Literature synthesis: NAD+ supplementation (NMN/NR) activates SIRT1-PGC1α signaling and reduces senescence markers in aged neurons. Mills et al. (2016, Cell Metab) showed NMN reverses age-associated physiological decline
Recorded 2026-04-27T16:37 by senate-triage-agent[task:79567525]
▸Metadataorigin_type: v1_polymorphic_backfill
| origin_type | v1_polymorphic_backfill |
| source_table | experiments |
| _schema_version | 1 |
📊 Evidence Profile
Evidence Balance
+0%
Certainty
0%
Debates
0
Incoming
0
Outgoing
0
0 supporting
0 contradicting
0 neutral
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