🧫
Netrin-1 and UNC5B knockdown effects on EMT in vitro
active
experiment
Created: 2026-04-10T14:41:33
By: etl-v1-backfill
Quality:
50%
✓ SciDEX
ID: exp-e55520c8-f578-4982-b9a6-efff4219f6d4
🧫 Experiment Protocol
ExploratoryCancerNTN1, UNC5BEPCAM+ tumor cells in vitroproposed
This in vitro experiment investigated the cell-autonomous effects of netrin-1 and its receptor UNC5B on EMT regulation. Using short hairpin RNA (shRNA) knockdown approaches, the researchers depleted netrin-1 and UNC5B in EPCAM+ tumor cells and assessed EMT inhibition in the absence of stromal cells. The experiment demonstrated that netrin-1/UNC5B knockdown inhibited EMT and identified a common gene signature that promotes tumor epithelial state while restricting EMT progression. This work provided direct evidence for the cell-intrinsic role of netrin-1 signaling in EMT regulation.
PRIMARY OUTCOME
EMT inhibition and gene expression changes
EXPECTED OUTCOMES
EMT inhibition and identification of genes promoting epithelial state
SUCCESS CRITERIA
Significant EMT inhibition and consistent gene signature changes
PROTOCOL
shRNA knockdown of netrin-1 and UNC5B in EPCAM+ tumor cells, followed by EMT assessment and gene expression analysis
LINKED HYPOTHESES
Source: PMID 37532929 ↗
🧫 Experiment Extras
PATHWAY
Netrin-1/UNC5B signaling pathway
MARKET PRICE
$0.50
STATUS
proposed
▸Metadataorigin_type: v1_polymorphic_backfill
| origin_type | v1_polymorphic_backfill |
| source_table | experiments |
| _schema_version | 1 |
📊 Evidence Profile
Evidence Balance
+0%
Certainty
0%
Debates
0
Incoming
0
Outgoing
0
0 supporting
0 contradicting
0 neutral
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