🖼
Figure 2: Gch1 overexpression and its downstream metabolites BH4/BH2 rescue from ferroptos...
active
paper figure
Created: 2026-04-21T12:28:25
By: paper_figures_pipeline
Quality:
50%
🔗 External
ID: paper-fig-31989025-2
Figure 2Figure 2
Gch1 overexpression and its downstream metabolites BH4/BH2 rescue from ferroptosis. (A) Survival of MF-dCas9-Gch1 (Gch1 OE) compared to empty vector MF control (control) cells and GCH1-IRES-Puro (GCH1 OE) cells compared to parental HT-1080 cells (parental), respectively, against inducers of cell death: Ferroptosis induced with 2 μM IKE and Gpx4–/– by using 1 μM tamoxifen with 10 μM α-tocopherol (αToc) rescue. Extrinsic apoptosis induced by 20 ng/mL tumor necrosis factor α (TNFα) with 10 μM z-VAD-FMK (zVAD) rescue. Intrinsic apoptosis induced by 20 μM doxorubicin (Doxo), 25 μM etoposide (Etop), and 12.5 μM colchicine (Colch). Necroptosis induced by 1 μg/mL lipopolysaccharide (LPS) cotreatment with 10 μM zVAD with 10 μM necrostatin-1 (Nec-1) rescue. (B) Dose response curves of BH2 and BH4 starting at 200 μM rescuing from ferroptosis in MF control and parental HT-1080 cells induced by 1 μM RSL3, 2 μM IKE, and Gpx4–/– by 1 μM tamoxifen. (C) Effect of BH2 and BH4 on lipid peroxidation induc
▸Metadata
| doi | |
| pmcid | PMC6978838 |
| _origin | {'url': 'https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6978838/figure/fig2/', 'type': 'external', 'tracked_at': '2026-04-21T19:28:25.610320'} |
| caption | Gch1 overexpression and its downstream metabolites BH4/BH2 rescue from ferroptosis. (A) Survival of MF-dCas9-Gch1 (Gch1 OE) compared to empty vector MF control (control) cells and GCH1-IRES-Puro (GCH1 |
| paper_id | 31989025 |
| image_url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6978838/figure/fig2/ |
| image_path | |
| description | |
| figure_label | Figure 2 |
| figure_number | 2 |
| _schema_version | 1 |
| source_strategy | pmc_api |
| entities_mentioned |
📊 Evidence Profile
Evidence Balance
+0%
Certainty
0%
Debates
0
Incoming
0
Outgoing
0
0 supporting
0 contradicting
0 neutral
Public annotations (0)Annotate on Hypothes.is →
No public annotations yet.