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Fig. 3: PDE4D5 expression is upregulated in human failing hearts and PDE4D5 induces hype...
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Created: 2026-04-22T18:08:31
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Fig. 3Figure 4
PDE4D5 expression is upregulated in human failing hearts and PDE4D5 induces hypertrophy and oxidative stress and inhibits mitophagy in NRVMs . A, Representative immunoblots and quantification of PDE4D and PDE4D variants (PDE4D5, PDE4D3, and PDE4D9) expression in left ventricle tissues from healthy donors (n = 3) and heart failure (HF) patients (n = 7). NRVMs were infected with mCherry-PDE4D5 adenovirus (Ad-PDE4D5) or control adenovirus (Ad-Ctrl) for 48 h (B through D ). B, The protein expressions of PDE4D and hypertrophy marker gene, ANP in NRVMs; n = 6 independent experiments. C, Immunofluorescence staining of α-actinin (green) and DAPI (blue) in NRVMs and quantification of myocytes area; n = 24 cells from 3 independent experiments. Scale bar, 20 μm. D, DCFH-DA staining and quantification of ROS levels in NRVMs; n = 5 independent experiments. Scale bar, 200 μm. E, MitoSOX staining and quantification of mitochondrial ROS levels in NRVMs infected with GFP-N-terminal-PDE4D5 a
▸Metadata
| doi | 10.1016/j.redox.2025.103563 |
| pmcid | PMC11909752 |
| _origin | {'url': 'https://www.ebi.ac.uk/europepmc/articles/PMC11909752/bin/gr3.jpg', 'type': 'external', 'tracked_at': '2026-04-23T01:08:31.300161'} |
| caption | PDE4D5 expression is upregulated in human failing hearts and PDE4D5 induces hypertrophy and oxidative stress and inhibits mitophagy in NRVMs . A, Representative immunoblots and quantification of PDE4 |
| paper_id | 9a10f9ee-2201-47b2-a4ca-b169666a53d9 |
| image_url | https://www.ebi.ac.uk/europepmc/articles/PMC11909752/bin/gr3.jpg |
| image_path | |
| description | |
| figure_label | Fig. 3 |
| figure_number | 4 |
| _schema_version | 1 |
| source_strategy | pmc_api |
| entities_mentioned |
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