🧫
Transcriptome sequencing of cardiac fibroblasts in TFEB overexpressing mice
active
experiment
Created: 2026-04-10T22:46:10
By: etl-v1-backfill
Quality:
50%
✓ SciDEX
ID: exp-41a0b4b1-9406-4ed5-b496-fb18149232f6
🧫 Experiment Protocol
Exploratorymyocardial infarctionTFEBR26-LSL-TFEB+/+; Acta2-cre mice and R26-LSL-TFEB+/+ control miceproposed
Following myocardial infarction induction, transcriptome sequencing was performed on cardiac fibroblasts isolated from R26-LSL-TFEB+/+; Acta2-cre mice (TFEB overexpressing) and control R26-LSL-TFEB+/+ mice. The study aimed to investigate how TFEB modulates the gene expression profile of cardiac fibroblasts after myocardial injury. Differential gene expression analysis revealed that TFEB modulated expression of genes associated with cardiac fibroblast transformation. Pathway analysis showed significant enrichment in extracellular matrix receptor interaction and focal adhesion pathways. The results demonstrated that TFEB overexpression leads to broad transcriptional changes in cardiac fibroblasts, particularly affecting genes involved in ECM signaling and cellular adhesion processes.
PRIMARY OUTCOME
differential gene expression patterns in cardiac fibroblasts
EXPECTED OUTCOMES
TFEB would modulate gene expression in cardiac fibroblasts affecting fibrosis-related pathways
SUCCESS CRITERIA
Significant differential gene expression and pathway enrichment in TFEB overexpressing cells
PROTOCOL
Myocardial infarction induction, cardiac fibroblast isolation, RNA extraction, transcriptome sequencing, differential gene expression analysis using R software
LINKED HYPOTHESES
Source: PMID 41935359 ↗
🧫 Experiment Extras
PATHWAY
extracellular matrix receptor interaction, focal adhesion
MARKET PRICE
$0.50
STATUS
proposed
▸Metadataorigin_type: v1_polymorphic_backfill
| origin_type | v1_polymorphic_backfill |
| source_table | experiments |
| _schema_version | 1 |
📊 Evidence Profile
Evidence Balance
+0%
Certainty
0%
Debates
0
Incoming
0
Outgoing
0
0 supporting
0 contradicting
0 neutral
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