Exploratory experiment designed to discover new patterns targeting TFEB in R26-LSL-TFEB+/+; Acta2-cre mice and R26-LSL-TFEB+/+ control mice. Primary outcome: differential gene expression patterns in cardiac fibroblasts
Following myocardial infarction induction, transcriptome sequencing was performed on cardiac fibroblasts isolated from R26-LSL-TFEB+/+; Acta2-cre mice (TFEB overexpressing) and control R26-LSL-TFEB+/+ mice. The study aimed to investigate how TFEB modulates the gene expression profile of cardiac fibroblasts after myocardial injury. Differential gene expression analysis revealed that TFEB modulated expression of genes associated with cardiac fibroblast transformation. Pathway analysis showed significant enrichment in extracellular matrix receptor interaction and focal adhesion pathways. The results demonstrated that TFEB overexpression leads to broad transcriptional changes in cardiac fibroblasts, particularly affecting genes involved in ECM signaling and cellular adhesion processes.
Myocardial infarction induction, cardiac fibroblast isolation, RNA extraction, transcriptome sequencing, differential gene expression analysis using R software
TFEB would modulate gene expression in cardiac fibroblasts affecting fibrosis-related pathways
Significant differential gene expression and pathway enrichment in TFEB overexpressing cells
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