🧫
Trehalose analog testing for autophagy induction
active
experiment
Created: 2026-04-06T12:29:04
By: etl-v1-backfill
Quality:
50%
✓ SciDEX
ID: exp-eb6c3b7d-b813-471c-90bc-79b65b7b6dde
🧫 Experiment Protocol
Exploratorymotoneuron degenerationTFEBNSC34 neuroblastoma x spinal cord cellsproposed
This experiment tested whether the autophagy-inducing effects of trehalose could be replicated by trehalase-resistant analogs, specifically melibiose and lactulose. This approach was designed to determine whether trehalose's effects are dependent on its metabolism by trehalase or are due to its direct molecular properties. The researchers measured similar endpoints as with trehalose, including lysosomal changes, TFEB activation, and autophagy gene expression.
PRIMARY OUTCOME
autophagy induction and TFEB activation
EXPECTED OUTCOMES
Trehalose analogs should produce similar effects to trehalose, suggesting metabolism-independent mechanism
SUCCESS CRITERIA
Similar lysosomal changes and TFEB activation as seen with trehalose treatment
PROTOCOL
Treatment with trehalose analogs (melibiose and lactulose) followed by assessment of lysosomal changes, TFEB nuclear translocation, and autophagy gene expression
LINKED HYPOTHESES
Source: PMID 30335591 ↗
🧫 Experiment Extras
PATHWAY
autophagy/lysosome pathway
MARKET PRICE
$0.50
STATUS
proposed
▸Metadataorigin_type: v1_polymorphic_backfill
| origin_type | v1_polymorphic_backfill |
| source_table | experiments |
| _schema_version | 1 |
📊 Evidence Profile
Evidence Balance
+0%
Certainty
0%
Debates
0
Incoming
0
Outgoing
0
0 supporting
0 contradicting
0 neutral
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