Exploratory experiment designed to discover new patterns targeting TFEB in NSC34 neuroblastoma x spinal cord cells. Primary outcome: autophagy induction and TFEB activation
This experiment tested whether the autophagy-inducing effects of trehalose could be replicated by trehalase-resistant analogs, specifically melibiose and lactulose. This approach was designed to determine whether trehalose's effects are dependent on its metabolism by trehalase or are due to its direct molecular properties. The researchers measured similar endpoints as with trehalose, including lysosomal changes, TFEB activation, and autophagy gene expression.
Treatment with trehalose analogs (melibiose and lactulose) followed by assessment of lysosomal changes, TFEB nuclear translocation, and autophagy gene expression
Trehalose analogs should produce similar effects to trehalose, suggesting metabolism-independent mechanism
Similar lysosomal changes and TFEB activation as seen with trehalose treatment
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