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Figure 4.: Direct effects of acetylcholine on DMG (A) Timeline for the generation of iPSC-d...
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Created: 2026-04-22T18:07:49
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Figure 4.Figure 4
Direct effects of acetylcholine on DMG (A) Timeline for the generation of iPSC-derived cholinergic with viral transduction (AAV-VAChTe1-ChRmine::eYFP or AAV-VAChTe1::eYFP) for co-culturing with glioma cells. (B) Confocal micrographs showing cholinergic motor neurons generated hiPSCs of a healthy 12-year-old male and co-cultured with DMG cells of an 8-year old male. NF-H/M, turquoise; H3K27M, white; scale bars, 50 μm. (C) Quantification of glioma cell proliferation (EdU + /H3K27M + ) when co-cultured with hiPSC-derived cholinergic motor neurons paired with in vitro optogenetic stimulation. One-way analysis of variance (ANOVA) with Tukey’s post hoc analysis; **** p < 0.0001, ** p < 0.01, ns: non-significant. Data = mean ± SEM; n = four independent experiments, each with three wells per condition; each data point represents the mean of three wells per condition for a given experiment. (D) Three-dimensional rendering illustrating presynaptic cholinergic neuron (NF-H/M: white) with pr
▸Metadata
| doi | 10.1016/j.cell.2025.05.031 |
| pmcid | PMC12396346 |
| _origin | {'url': 'https://www.ebi.ac.uk/europepmc/articles/PMC12396346/bin/nihms-2096866-f0005.jpg', 'type': 'external', 'tracked_at': '2026-04-23T01:07:49.643647'} |
| caption | Direct effects of acetylcholine on DMG (A) Timeline for the generation of iPSC-derived cholinergic with viral transduction (AAV-VAChTe1-ChRmine::eYFP or AAV-VAChTe1::eYFP) for co-culturing with glioma |
| paper_id | 640f626a-ad3e-4ccf-a806-fbe18cc83b81 |
| image_url | https://www.ebi.ac.uk/europepmc/articles/PMC12396346/bin/nihms-2096866-f0005.jpg |
| image_path | |
| description | |
| figure_label | Figure 4. |
| figure_number | 4 |
| _schema_version | 1 |
| source_strategy | pmc_api |
| entities_mentioned |
📊 Evidence Profile
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