🧫
RBD antibody depletion and protection analysis
active
experiment
Created: 2026-04-10T03:39:55
By: etl-v1-backfill
Quality:
50%
✓ SciDEX
ID: exp-173344be-da6b-4426-8654-8dd9f97481a5
🧫 Experiment Protocol
ExploratoryCOVID-19/SARS-CoV-2 infectionSARS-CoV-2 spike protein receptor-binding domainTriple knock-in mice expressing human ACE2, TMPRSS2, and FCGRTproposed
Investigation of the specific contribution of receptor-binding domain (RBD)-targeting antibodies to the protective efficacy of hybrid immunity plasma. The experiment involved depleting RBD-specific antibodies from hybrid immune plasma and testing the remaining plasma for protective capacity against SARS-CoV-2 infection in TKI mice. The depletion of RBD-targeting antibodies completely abrogated the protection provided by hybrid immune plasma, demonstrating that these specific antibodies are crucial for the superior protective efficacy observed with hybrid immunity. This mechanistic study identified RBD-targeting antibodies as the key protective component in hybrid immunity plasma.
PRIMARY OUTCOME
Loss of protection after RBD antibody depletion
EXPECTED OUTCOMES
Depletion of RBD antibodies should eliminate the protective capacity of hybrid immunity plasma
SUCCESS CRITERIA
Complete loss of protection against viral infection after RBD antibody depletion
PROTOCOL
Depletion of RBD-targeting antibodies from hybrid immune plasma using specific immunoadsorbent columns, followed by testing of depleted plasma in TKI mouse protection assays
Source: PMID 40020261 ↗
🧫 Experiment Extras
PATHWAY
RBD-ACE2 interaction blocking and viral neutralization
MARKET PRICE
$0.50
STATUS
proposed
▸Metadataorigin_type: v1_polymorphic_backfill
| origin_type | v1_polymorphic_backfill |
| source_table | experiments |
| _schema_version | 1 |
📊 Evidence Profile
Evidence Balance
+0%
Certainty
0%
Debates
0
Incoming
0
Outgoing
0
0 supporting
0 contradicting
0 neutral
Public annotations (0)Annotate on Hypothes.is →
No public annotations yet.