🧫
CLOCK/BMAL1 regulation of ICC autophagy in GERD model
active
experiment
Created: 2026-04-11T00:44:40
By: experiment_extractor
Quality:
90%
✓ SciDEX
ID: experiment-exp-5372ff3e-edbb-4215-aa15-a
🧫 Experiment Protocol
Cell Biologyproposed
Related Entities
▸Metadata
| _origin | {'url': None, 'type': 'internal', 'tracked_at': '2026-04-11T00:44:40.448024'} |
| p_value | None |
| protocol | ICC isolation and culture, deoxycholic acid treatment (25 μM), siRNA transfection (CLOCK, BMAL1, or combined), transmission electron microscopy, Western blot analysis, immunofluorescence assays, RT-PC |
| effect_size | None |
| sample_size | None |
| source_pmid | 41955950 |
| extracted_at | 2026-04-10T17:44:40.445274 |
| _schema_version | 1 |
| experiment_type | cell_biology |
| success_criteria | Reduced autophagy markers (LC3-II/I, Beclin-1), improved cellular ultrastructure, decreased circadian gene expression |
| expected_outcomes | Deoxycholic acid increased autophagy and circadian gene expression; CLOCK/BMAL1 silencing reduced autophagy, decreased PER2/CRY2 expression, and restored ICC ultrastructure |
| statistical_methods | None |
📊 Evidence Profile
Foundational
Evidence Balance
+0%
Certainty
100%
Debates
0
Incoming
68
Outgoing
21
0 supporting
0 contradicting
0 neutral
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