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Extended Data Fig. 3: Spp1 TdT reporter mouse development. (a) Design of the knock-in allele is show...
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Created: 2026-04-15T23:28:06
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ID: paper-fig-36747024-8
Extended Data Fig. 3Figure 8
Spp1 TdT reporter mouse development. (a) Design of the knock-in allele is shown, with an IRES and TdTomato (TdT) reporter inserted at the stop codon in exon 7 in the mouse Spp1 locus. Top, the mouse locus with locations of primers used for validation of the inserted construct and later genotyping for the reporter construct is shown. Below, schematic displays the upstream (5’HA) and downstream (3’HA) homology arms in blue; IRES in aqua; TdT reporter gene in red; and bovine growth hormone poly(A) region in yellow. (b) PCR primers used to confirm incorporation of construct are listed, with primer names based on genomic locations as shown in B. Gel shows appropriate size bands for incorporation of plasmid construct (lane 1), for left arm integration event (lane 2) and right arm integration event (lane 3). Primer pairs for plasmid backbone alone are negative (lanes 4 and 5). (c) Representative confocal image showing SPP1-TdT expression along GLUT1 + vessels in hippocampus of 3 mo
▸Metadata
| doi | |
| pmcid | PMC9991912 |
| _origin | {'url': 'https://www.ebi.ac.uk/europepmc/articles/PMC9991912/bin/41593_2023_1257_Fig8_ESM.jpg', 'type': 'external', 'tracked_at': '2026-04-15T23:28:06.305226'} |
| caption | Spp1 TdT reporter mouse development. (a) Design of the knock-in allele is shown, with an IRES and TdTomato (TdT) reporter inserted at the stop codon in exon 7 in the mouse Spp1 locus. Top, the mou |
| paper_id | 36747024 |
| image_url | https://www.ebi.ac.uk/europepmc/articles/PMC9991912/bin/41593_2023_1257_Fig8_ESM.jpg |
| image_path | |
| description | |
| figure_label | Extended Data Fig. 3 |
| figure_number | 8 |
| _schema_version | 1 |
| source_strategy | pmc_api |
| entities_mentioned |
📊 Evidence Profile
Evidence Balance
+0%
Certainty
0%
Debates
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Incoming
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Outgoing
1
0 supporting
0 contradicting
0 neutral
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