🧫
TUG C-terminal product nuclear localization and PPARγ binding
active
experiment
Created: 2026-04-06T12:33:38
By: etl-v1-backfill
Quality:
50%
✓ SciDEX
ID: exp-12ee7d80-bab1-4e11-ba5d-1ec524302607
🧫 Experiment Protocol
ExploratoryMetabolic regulationTug (Aspscr1)Cell culture and mouse tissueproposed
Investigation of the mechanism by which the TUG C-terminal cleavage product enters the nucleus and interacts with PPARγ and PGC-1α. This study demonstrated that after insulin-stimulated cleavage and GLUT4 release, the TUG C-terminal fragment translocates to the nucleus where it binds to transcriptional regulators to control gene expression programs involved in lipid oxidation and thermogenesis.
PRIMARY OUTCOME
Nuclear localization and protein-protein interactions
EXPECTED OUTCOMES
TUG C-terminal product localizes to nucleus and binds PPARγ/PGC-1α
SUCCESS CRITERIA
Demonstration of nuclear localization and specific protein interactions
PROTOCOL
Subcellular fractionation, immunofluorescence microscopy, co-immunoprecipitation, chromatin immunoprecipitation
Source: PMID 33686286 ↗
🧫 Experiment Extras
PATHWAY
PPARγ/PGC-1α transcriptional regulation
MARKET PRICE
$0.50
STATUS
proposed
▸Metadataorigin_type: v1_polymorphic_backfill
| origin_type | v1_polymorphic_backfill |
| source_table | experiments |
| _schema_version | 1 |
📊 Evidence Profile
Evidence Balance
+0%
Certainty
0%
Debates
0
Incoming
0
Outgoing
0
0 supporting
0 contradicting
0 neutral
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