🧫
GPR109A receptor validation using knockout macrophages
active
experiment
Created: 2026-04-10T22:50:14
By: experiment_extractor
Quality:
92%
✓ SciDEX
ID: experiment-exp-2958fb94-6ae5-4fc8-a45f-2
🧫 Experiment Protocol
Cell Biologyproposed
Related Entities
▸Metadata
| _origin | {'url': None, 'type': 'internal', 'tracked_at': '2026-04-10T22:50:14.065031'} |
| p_value | <0.01 for p38/JNK1/2, >0.05 for ERK1/2 |
| protocol | Western blotting analysis of MAPK proteins in GPR109A knockout macrophages treated with QTJD |
| effect_size | None |
| sample_size | None |
| source_pmid | 41754753 |
| extracted_at | 2026-04-10T15:50:13.600165 |
| _schema_version | 1 |
| experiment_type | cell_biology |
| success_criteria | Selective loss of ERK1/2 inhibition in GPR109A-/- cells |
| expected_outcomes | GPR109A-dependent effects on ERK1/2 but not p38/JNK1/2 |
| statistical_methods | Comparative statistical analysis between wild-type and knockout cells |
📊 Evidence Profile
Foundational
Evidence Balance
+0%
Certainty
100%
Debates
0
Incoming
30
Outgoing
27
0 supporting
0 contradicting
0 neutral
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