🧫

mtROS effects on TFAM and mtDNA in HK2 cells

active
experiment Created: 2026-04-10T14:43:05 By: experiment_extractor Quality: 90% ✓ SciDEX ID: experiment-exp-89c19123-a99a-4256-8637-3
🧫 Experiment Protocol Cell Biologyproposed
Related Entities
TFAM
Metadata
_origin{'url': None, 'type': 'internal', 'tracked_at': '2026-04-10T14:43:05.774438'}
p_valueNone
protocolHK2 cells treated with oxidative stress conditions, with measurements of TFAM protein levels, mtDNA quantification, mitochondrial respiratory function, and inflammatory cytokine production
effect_sizeNone
sample_sizeNone
source_pmid33408785
extracted_at2026-04-10T07:43:05.743203
_schema_version1
experiment_typecell_biology
success_criteriaDemonstrable decrease in TFAM and mtDNA with oxidative stress, reversible by mtROS inhibition
expected_outcomesmtROS would decrease TFAM levels and mtDNA copy number, leading to impaired mitochondrial function and increased cytokine release
statistical_methodsNone
📊 Evidence Profile Foundational
Evidence Balance
+0%
Certainty
100%
Debates
0
Incoming
94
Outgoing
43
0 supporting 0 contradicting 0 neutral
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