Synthetic Biology Approach: Designer Mitochondrial Export Systems

graph TD
    A["Synthetic Fusion<br/>Proteins"] --> B["Mitochondrial<br/>Targeting Signals"]
    A --> C["Cell Surface<br/>Export Domains"]
    B --> D["Healthy Donor<br/>Mitochondria"]
    C --> E["Extracellular<br/>Vesicle Formation"]
    D --> F["Enhanced Mitochondrial<br/>Packaging"]
    E --> G["Targeted Vesicle<br/>Release"]
    F --> G
    G --> H["Recipient Neuron<br/>Uptake"]
    H --> I["Mitochondrial<br/>Integration"]
    I --> J["Restored ATP<br/>Production"]
    I --> K["Improved Ca2+<br/>Homeostasis"]
    I --> L["Reduced ROS<br/>Generation"]
    J --> M["Neuronal Function<br/>Recovery"]
    K --> M
    L --> M
    N["Neurodegeneration<br/>Pathology"] --> O["Mitochondrial<br/>Dysfunction"]
    O --> P["Therapeutic<br/>Intervention"]
    P --> M

    classDef normal fill:#4fc3f7,color:#0d0d1a
    classDef therapeutic fill:#81c784,color:#0d0d1a
    classDef pathology fill:#ef5350,color:#0d0d1a
    classDef outcome fill:#ffd54f,color:#0d0d1a
    classDef molecular fill:#ce93d8,color:#0d0d1a

    class D,H,I,J,K,L normal
    class A,E,F,G,P therapeutic
    class N,O pathology
    class M outcome
    class B,C molecular