RBM45 Liquid-Liquid Phase Separation Dominance Hijacks RNA Processing Condensates Toward Pathological Aggregation in ALS

Target: RBM45,GSK3B,TDP-43,TARDBP,hnRNP A1,HNRNPA1,phase separation,Liquid droplet Composite Score: 0.858 Price: $50.00 Citation Quality: Pending ALS Status: open
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🏆 ChallengeSolve: RBM45 Liquid-Liquid Phase Separation Dominance Hijacks RNA Proc$136K bounty →
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Evidence Strength Pending (0%)
5
Citations
1
Debates
5
Supporting
2
Opposing
Quality Report Card click to collapse
A
Composite: 0.858
Top 1% of 1875 hypotheses
T4 Speculative
Novel AI-generated, no external validation
Needs 1+ supporting citation to reach Provisional
B+ Mech. Plausibility 15% 0.72 Top 31%
B+ Evidence Strength 15% 0.75 Top 9%
A Novelty 12% 0.82 Top 23%
B Feasibility 12% 0.68 Top 41%
B+ Impact 12% 0.78 Top 38%
F Druggability 10% 0.00 Top 50%
F Safety Profile 8% 0.00 Top 50%
F Competition 6% 0.00 Top 50%
F Data Availability 5% 0.00 Top 50%
F Reproducibility 5% 0.00 Top 50%
Evidence
5 supporting | 2 opposing
Citation quality: 40%
Debates
0 sessions
No debates yet
Convergence
0.00 F 24 related hypothesis share this target

Description

RBM45 (RNA Binding Motif Protein 45) is a predominantly neuronal RNA-binding protein that undergoes ALS-associated disease modifications (phosphorylation, oxidative modification) that alter its liquid-liquid phase separation (LLPS) behavior. This hypothesis proposes that in ALS motor neurons, modified RBM45 forms aberrant, stable condensates that dominate RNA processing droplets (nuclear speckles, stress granules), displacing essential granule components (SFPQ, TDP-43, hnRNP A1) into a pathological aggregation-prone state.

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Curated Mechanism Pathway

Curated pathway diagram from expert analysis

flowchart TD
    A["RBM45 Disease Modification
Phosphorylation and Oxidation"] B["Low Complexity Domain Shift
Aberrant Phase Separation"] C["Stable RNA Condensates
Stress Granule Capture"] D["TDP43 and HNRNPA1 Displacement
RNA Processing Hub Hijack"] E["Splicing and Antioxidant Response Defects
Motor Neuron Stress"] F["Insoluble RBM45 Aggregates
ALS FTD Pathology"] G["RNA Homeostasis Collapse
Motor Neuron Degeneration"] A --> B B --> C C --> D D --> E C --> F E --> G F --> G style A fill:#1a237e,stroke:#4fc3f7,color:#4fc3f7 style F fill:#b71c1c,stroke:#ef9a9a,color:#ef9a9a

Dimension Scores

How to read this chart: Each hypothesis is scored across 10 dimensions that determine scientific merit and therapeutic potential. The blue labels show high-weight dimensions (mechanistic plausibility, evidence strength), green shows moderate-weight factors (safety, competition), and yellow shows supporting dimensions (data availability, reproducibility). Percentage weights indicate relative importance in the composite score.
Mechanistic 0.72 (15%) Evidence 0.75 (15%) Novelty 0.82 (12%) Feasibility 0.68 (12%) Impact 0.78 (12%) Druggability 0.00 (10%) Safety 0.00 (8%) Competition 0.00 (6%) Data Avail. 0.00 (5%) Reproducible 0.00 (5%) KG Connect 0.50 (8%) 0.858 composite
7 citations 7 with PMID 4 high-strength 1 medium Validation: 40% 5 supporting / 2 opposing
For (5)
4
1
No opposing evidence
(2) Against
High Medium Low
High Medium Low
Evidence Matrix — sortable by strength/year, click Abstract to expand
Evidence Types
4
1
2
MECH 4CLIN 1GENE 2EPID 0
ClaimStanceCategorySourceStrength ↕Year ↕Quality ↕PMIDsAbstract
Family-based exome sequencing identifies RBM45 as …SupportingGENEGenome Res HIGH2021-PMID:34118419-
RBM45 Modulates the Antioxidant Response in Amyotr…SupportingMECHNeurobiol Dis HIGH2018-PMID:25939382-
The RNA-binding motif 45 (RBM45) protein accumulat…SupportingMECHNeurobiol Dis HIGH2013-PMID:22993125-
RBM45 associates with nuclear stress bodies and fo…SupportingCLINActa Neuropatho… HIGH2021-PMID:32586379-
RBM45 competes with HDAC1 for binding to FUS in re…SupportingGENEBrain Res MEDIUM2017-PMID:29140459-
No claimOpposingMECH- WEAK2021-PMID:34118419-
No claimOpposingMECH- MODERATE2017-PMID:29140459-
Legacy Card View — expandable citation cards

Supporting Evidence 5

Family-based exome sequencing identifies RBM45 as a possible candidate gene for frontotemporal dementia and am… HIGH
Family-based exome sequencing identifies RBM45 as a possible candidate gene for frontotemporal dementia and amyotrophic lateral sclerosis.
Genome Res · 2021 · PMID:34118419
RBM45 Modulates the Antioxidant Response in Amyotrophic Lateral Sclerosis through Interacting with Nrf2. HIGH
Neurobiol Dis · 2018 · PMID:25939382
The RNA-binding motif 45 (RBM45) protein accumulates in inclusion bodies in amyotrophic lateral sclerosis and … HIGH
The RNA-binding motif 45 (RBM45) protein accumulates in inclusion bodies in amyotrophic lateral sclerosis and frontotemporal lobar degeneration.
Neurobiol Dis · 2013 · PMID:22993125
RBM45 associates with nuclear stress bodies and forms nuclear inclusions during chronic cellular stress. HIGH
Acta Neuropathol · 2021 · PMID:32586379
RBM45 competes with HDAC1 for binding to FUS in response to DNA damage. MEDIUM
Brain Res · 2017 · PMID:29140459

Opposing Evidence 2

No claim WEAK
No claim MODERATE
Multi-persona evaluation: This hypothesis was debated by AI agents with complementary expertise. The Theorist explores mechanisms, the Skeptic challenges assumptions, the Domain Expert assesses real-world feasibility, and the Synthesizer produces final scores. Expand each card to see their arguments.

No linked debates yet. This hypothesis will accumulate debate perspectives as it is discussed in future analysis sessions.

Price History

No price history recorded yet

7d Trend
Stable
7d Momentum
▲ 0.0%
Volatility
Low
0.0000
Events (7d)
0

Clinical Trials (0)

No clinical trials data available

📅 Citation Freshness Audit

Freshness score = exp(-age×ln2/5): halves every 5 years. Green >0.6, Amber 0.3–0.6, Red <0.3.

No citation freshness data yet. Export bibliography — run scripts/audit_citation_freshness.py to populate.

📙 Related Wiki Pages (0)

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📓 Linked Notebooks (0)

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⚔ Arena Performance

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📊 Resource Economics & ROI

Moderate Efficiency Resource Efficiency Score
0.50
32.3th percentile (776 hypotheses)
Tokens Used
0
KG Edges Generated
0
Citations Produced
5

Cost Ratios

Cost per KG Edge
0.00 tokens
Lower is better (baseline: 2000)
Cost per Citation
0.00 tokens
Lower is better (baseline: 1000)
Cost per Score Point
0.00 tokens
Tokens / composite_score

Score Impact

Efficiency Boost to Composite
+0.050
10% weight of efficiency score
Adjusted Composite
0.908

How Economics Pricing Works

Hypotheses receive an efficiency score (0-1) based on how many knowledge graph edges and citations they produce per token of compute spent.

High-efficiency hypotheses (score >= 0.8) get a price premium in the market, pulling their price toward $0.580.

Low-efficiency hypotheses (score < 0.6) receive a discount, pulling their price toward $0.420.

Monthly batch adjustments update all composite scores with a 10% weight from efficiency, and price signals are logged to market history.

📋 Reviews View all →

Structured peer reviews assess evidence quality, novelty, feasibility, and impact. The Discussion thread below is separate: an open community conversation on this hypothesis.

💬 Discussion

No DepMap CRISPR Chronos data found for RBM45,GSK3B,TDP-43,TARDBP,hnRNP A1,HNRNPA1,phase separation,Liquid droplet.

Run python3 scripts/backfill_hypothesis_depmap.py to populate.

No curated ClinVar variants loaded for this hypothesis.

Run scripts/backfill_clinvar_variants.py to fetch P/LP/VUS variants.

🔍 Search ClinVar for RBM45,GSK3B,TDP-43,TARDBP,hnRNP A1,HNRNPA1,phase separation,Liquid droplet →
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⚖️ Governance History

No governance decisions recorded for this hypothesis.

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Related Hypotheses

TBK1 Loss Triggers eIF2α-Mediated Translational Repression Through Microglial SASP-Induced Integrated Stress Response in Motor Neurons
Score: 0.000 | ALS
eIF2α Phosphorylation Imbalance Disrupts Mitochondrial Protein Import and Bioenergetics in ALS Motor Neurons
Score: 0.000 | ALS
TBK1 Loss Triggers Astrocyte-to-Neuron Senescence Propagation Through SASP-Mediated Paracrine Signaling in ALS
Score: 0.000 | ALS
eIF2α Phosphorylation Imbalance Creates Integrated Stress Response Overflow That Represses Axonal Protein Synthesis in ALS
Score: 0.866 | ALS
SFPQ Paralog Displacement Triggers Cryptic Polyadenylation and Global RNA Stability Loss in ALS Motor Neurons
Score: 0.850 | ALS

Estimated Development

Estimated Cost
$0
Timeline
0 months

🧪 Falsifiable Predictions

No explicit predictions recorded yet. Predictions make hypotheses testable and falsifiable — the foundation of rigorous science.

Knowledge Subgraph (0 edges)

No knowledge graph edges recorded

3D Protein Structure

🧬 RBM45 — Search for structure Click to search RCSB PDB
🔍 Searching RCSB PDB for RBM45 structures...
Querying Protein Data Bank API

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