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A 3D-Printed Pulsatile Shear Stress Platform for Studying Endothelial Cell Mechanobiology.
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A 3D-Printed Pulsatile Shear Stress Platform for Studying Endothelial Cell Mechanobiology.
Romanov V, Li JV, Zhao F, Cheng D, Martinac B et al.
Abstract
The tools for subjecting cells to high-magnitude shear stress within conventional cell culture dishes and well plates on an orbital shaking platform have remained unchanged for the past 30 years. Here, we develop a pipeline for creating custom cell culture dishes and well plates of arbitrary size and complexity using 3D printing technology. We describe two methods: direct 3D printing and reversible bonding of ACLAR film to 3D-printed structures. We show that the custom chambers support alignment...
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