Fig. 2: SPP1 is expressed by PVMs and fibroblasts. a – c , Representative images of Spp...

SPP1 is expressed by PVMs and fibroblasts. a – c , Representative images of Spp1 mRNA expression juxtaposed to GLUT1 + vasculature, colocalizing with pan-PVM markers Cd163 ( a ), CD206 ( b ) and PVF ( Pdgfra + ) ( c ) in 6-month App NL-F SLM as characterized by smFISH-IHC. Scale bar represents 10 µm. Data are representative of four App NL-F mice examined over two independent experiments. d – f , Representative FACS plots to identify PVMs (CX3CR1 + CD45 + CD11b + CD206 + ( d ), microglia CX3CR1 high CD45 + CD11b + ( e ) or gated on total TdT expressing cells isolated from Spp1 TdT hippocampal homogenates and quantification ( f )). One datapoint represents one cell type per mouse (PVM, PVF and microglia) pooled from n  = 7 mice examined over two independent experiments ( f ). P values from one-way ANOVA, Bonferroni’s multiple comparison test. g , Three-dimensional reconstruction of CD206 + PVMs expressing SPP1-Td along GLUT1 + vessels in SLM from Spp1 TdT mice. Scal

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