Adrenaline restores Bmal1 transcriptional rhythms dampened by SARS-CoV-2 infection in Cthrc1-positive pulmonary fibroblasts derived from the Nile grass rat.

Disturbances in sleep and circadian rhythms are observed as sequelae of COVID-19 syndrome. However, the pathogenetic mechanisms remain largely unknown. Here, we established a fibroblast cell line (GrLBmal1-luc) from the lungs of diurnal Nile grass rats stably expressing the Bmal1-luciferase reporter gene to investigate peripheral clock regulation. First, endogenous receptor expression was screened with quantitative RT-PCR, immunohistochemistry, Ca2+ imaging, and Bmal1-luciferase reporter in GrLB...