Glycosaminoglycan Template Disruption Approach

graph TD
    A["HSPG2 perlecan<br/>extracellular matrix"] --> B["Heparan sulfate chains<br/>polyanionic surface"]
    B --> C["Electrostatic attraction<br/>of amyloidogenic proteins"]
    C --> D["Alpha-synuclein<br/>monomer binding"]
    C --> E["Amyloid-beta<br/>monomer binding"]
    C --> F["Tau protein<br/>monomer binding"]
    
    D --> G["Local concentration<br/>micromolar range"]
    E --> G
    F --> G
    
    G --> H["Nucleation template<br/>0.5-1.0 nm sulfate spacing"]
    H --> I["Conformational catalysis<br/>beta-sheet formation"]
    I --> J["Cross-beta amyloid<br/>fibril assembly"]
    
    J --> K["Cross-seeding between<br/>different amyloid species"]
    K --> L["Synaptic dysfunction<br/>and neuroinflammation"]
    L --> M["Progressive neuronal<br/>death and degeneration"]
    
    N["Glycosaminoglycan lyases<br/>targeted delivery"] -->|"disrupts"| H
    O["Competitive GAG inhibitors<br/>heparin mimetics"] -->|"blocks"| C
    
    N --> P["Preserved normal<br/>GAG functions"]
    O --> P

    classDef normal fill:#4fc3f7,color:#0d0d1a
    classDef therapeutic fill:#81c784,color:#0d0d1a
    classDef pathology fill:#ef5350,color:#0d0d1a
    classDef outcome fill:#ffd54f,color:#0d0d1a
    classDef molecular fill:#ce93d8,color:#0d0d1a
    
    class A,B,G,H normal
    class N,O,P therapeutic
    class J,K,L,M pathology
    class I outcome
    class C,D,E,F molecular