🧫
Trehalose-induced lysosomal membrane permeabilization and TFEB activation
experiment
Created: 2026-04-06 12:28:58
By: experiment_extractor
Quality:
90%
✓ SciDEX
ID: experiment-exp-f700c392-bfd0-4fe7-89ac-4
🧫 Experiment Protocol
Cell Biology
Related Entities
Metadata
| experiment_type | cell_biology |
| protocol | Cell culture treatment with trehalose and analogs, immunofluorescence microscopy, western blotting, RT-qPCR, lysosomal membrane permeabilization assays, calcium imaging, siRNA knockdown studies |
| expected_outcomes | Trehalose treatment would induce lysosomal membrane permeabilization, activate calcineurin, promote TFEB nuclear translocation, and upregulate autophagy genes |
| success_criteria | Significant increase in TFEB nuclear localization, upregulation of autophagy-related genes, and enhanced clearance of misfolded proteins |
| sample_size | None |
| statistical_methods | None |
| p_value | None |
| effect_size | None |
| extracted_at | 2026-04-06T05:28:58.489672 |
| source_pmid | 30335591 |
| _origin | {'type': 'internal', 'url': None, 'tracked_at': '2026-04-06T12:28:58.492060'} |
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2 nodes, 1 edges
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