🧫

Trehalose-induced lysosomal changes and TFEB activation

experiment Created: 2026-04-06 12:29:04 By: experiment_extractor Quality: 90% ✓ SciDEX ID: experiment-exp-b939a41c-0445-4658-bdaf-1
🧫 Experiment Protocol Cell Biology
🧫 View Full Experiment Page →
Related Entities
TFEB
Metadata
experiment_typecell_biology
protocolCells treated with trehalose, followed by immunofluorescence analysis for TFEB localization and lysosomal markers, biochemical assays for PPP3/calcineurin activity, and measurement of lysosomal membra
expected_outcomesTrehalose treatment should induce lysosomal changes leading to TFEB activation and autophagy upregulation
success_criteriaSignificant TFEB nuclear translocation, lysosomal enlargement, and increased autophagy gene expression
sample_sizeNone
statistical_methodsNone
p_valueNone
effect_sizeNone
extracted_at2026-04-06T05:29:04.356050
source_pmid30335591
_origin{'type': 'internal', 'url': None, 'tracked_at': '2026-04-06T12:29:04.358509'}
🌍 Provenance Graph 2 nodes, 1 edges

derives from (1)

Linked Artifacts (315)
derives_from📄Trehalose induces autophagy via lysosomal-mediated TFEB acti100%
related🧫TFEB silencing and misfolded protein degradation100%
related🧫Transcriptome sequencing of cardiac fibroblasts in TFEB over100%
related🧫Functional validation of trehalose effects on misfolded prot100%
related🧫Trehalose analog testing for autophagy induction100%
related🧫Trehalose-induced lysosomal membrane permeabilization and TF100%
related🧪The Mitochondrial-Lysosomal Metabolic Coupling Dysfunction100%
related🧪TFEB-PGC1α Mitochondrial-Lysosomal Decoupling100%
mentions📖entities-tfeb80%
mentions📖TFEB80%
mentions📖TFEB-Mediated Lysosomal Biogenesis80%
mentions📖TFEB Activators for Parkinson's Disease80%
mentions📖TFEB Protein (Transcription Factor EB)80%
mentions📖TFEB Protein80%
mentions📖TFEB Activators in Neurodegeneration80%
mentions📖tfeb-activators-neurodegeneration80%
related📖entities-tfeb72%
related📖TFEB72%
related📖TFEB-Mediated Lysosomal Biogenesis72%
related📖TFEB Activators for Parkinson's Disease72%
related📖TFEB Protein (Transcription Factor EB)72%
related📖TFEB Protein72%
related📖TFEB Activators in Neurodegeneration72%
related📖tfeb-activators-neurodegeneration72%
mentions📖TFEB Autophagy Pathway70%
mentions📖TFEB Signaling in Neurodegeneration70%
mentions📖TFEB Activator Therapies for Neurodegenerative Diseases70%
related📖TFEB Autophagy Pathway63%
related📖TFEB Signaling in Neurodegeneration63%
related📖TFEB Activator Therapies for Neurodegenerative Diseases63%
mentions📖Autophagy60%
mentions📖researchers60%
mentions📖autophagy60%
related🧫TFEB silencing and misfolded protein degradation60%
related🧫Transcriptome sequencing of cardiac fibroblasts in TFEB over60%
related🧪The Mitochondrial-Lysosomal Metabolic Coupling Dysfunction60%
related🧪TFEB-PGC1α Mitochondrial-Lysosomal Decoupling60%
related📖autophagy55%
related📖Autophagy54%
related📖researchers54%
related📖autophagy54%
mentions📖Combination Logic Ideas — Cross-Modality Pairings50%
mentions📖endosomal-lysosomal-cbs50%
mentions📖mTOR Signaling in Parkinson's Disease50%
mentions📖Autophagy Inducers in Neurodegeneration50%
mentions📖Proteostasis Therapy CBS PSP50%
mentions📖Section 102: Proteostasis Network Dysregulation in CBS/PSP50%
mentions📖Novel Therapy Index50%
mentions📖Alpha-Synuclein Clearance Mechanisms50%
mentions📖rapamycin-tauopathy50%
mentions📖autophagy-lysosomal-ad50%
mentions📖Endosomal-Lysosomal Pathway in Neurodegeneration50%
mentions📖PSP Lysosomal Dysfunction and Autophagy Impairment50%
mentions📖CBS/PSP Treatment Rankings50%
mentions📖Section 194: Advanced Mitochondrial Dynamics and Biogenesis 50%
mentions📖Proteostasis Triad Pulses: ISR + Autophagy + Chaperone Induc50%
mentions📖Autophagy Enhancers for PD50%
mentions📖Section 204: Advanced Proteostasis and Protein Quality Contr50%
mentions📖autophagy-lysosomal-impairment-comparison50%
mentions📖Exercise-BDNF-Mitochondrial Resilience Hypothesis in Parkins50%
mentions📖Section 194: Advanced Mitochondrial Dynamics and Biogenesis 50%
mentions📖Autophagy-Proteostasis Dual Activation50%
mentions📖Autophagy-Lysosomal Pathway in Parkinson's Disease50%
mentions📖mTOR Signaling in 4R-Tauopathies50%
mentions📖Autophagy-Enhancing Therapies50%
mentions📖Alpha-Synuclein Autophagy Clearance50%
mentions📖Autophagy-Lysosomal Pathway50%
mentions📖Lipid Droplet-Lysosome Axis Dysfunction Hypothesis in Parkin50%
mentions📖Autophagy Failure Comparison -- AD/PD/ALS/FTD/HD50%
mentions📖Autophagy-Lysosomal Impairment Across Neurodegenerative Dise50%
mentions📖autophagy-lysosome-dysfunction50%
mentions📖Autophagy-Lysosomal Impairment Comparison — AD/PD/ALS/FTD/HD50%
mentions📖Autophagy Enhancement for Tauopathy50%
mentions📖Autophagy Molecular Regulation in Neurodegeneration50%
mentions📖cbs-psp-daily-action-plan50%
mentions📖Mitophagy Gate Therapy: PINK1/Parkin + TFEB Priming50%
mentions📖Autophagy-Impaired Neurons50%
mentions📖Autophagy-Lysosomal Dysfunction Neurons50%
mentions📖Neural Circuits in Progressive Supranuclear Palsy50%
mentions📖MDS 2026: Neuroprotection and Neurorestorative Strategies fo50%
mentions📖LRRK2/GBA Mutation Carrier Resilience — Why Some Carriers Ne50%
mentions📖TMEM106B Haplotype as Genetic Modifier in FTD — Mechanism an50%
mentions📖ESCRT-III Neuroprotection Therapy for Neurodegeneration50%
mentions📖Mitochondrial Dynamics Modulation Therapy50%
mentions📖TMEM175 Lysosomal Modulation Therapy50%
mentions📖Alpha-Synuclein ESCRT-III Inhibition Mechanism50%
mentions📖alpha-synuclein-LRRK2 Crosstalk50%
mentions📖Autophagy-Lysosome Pathway in Neurodegeneration50%
mentions📖becn1-autophagy-initiation-neurodegeneration-causal-chain50%
mentions📖DNAJC13→Endosomal Dysfunction→PD Causal Chain50%
mentions📖GBA and Lysosomal Function in Parkinson's Disease50%
mentions📖GBA Gene Mutations and Parkinson's Disease Risk50%
mentions📖HTT Gene-Mechanism-Therapy Causal Chain — Huntington's 50%
mentions📖Lipophagy Pathway in Neurodegeneration50%
mentions📖Macroautophagy50%
mentions📖Mitochondria in Neurodegeneration50%
mentions📖Peroxisome Dysfunction in Neurodegeneration50%
mentions📖Protein Aggregation and Misfolding in Neurodegeneration50%
mentions📖Protein Quality Control Network50%
mentions📖Therapeutic Synergy and Combination Approach Rankings50%
mentions📖Cross-Disease Therapeutic Targets in 4R-Tauopathies50%
mentions📖Section 194: Advanced Membrane Trafficking and Vesicle Dynam50%
mentions📖Section 189: Advanced Autophagy-Endolysosomal Pathway Therap50%
mentions📖mTOR Signaling in Autophagy and Lysosomal Function50%
mentions📖Metabolic-Energetic Restoration: GLP-1 + TFEB Activator Comb50%
mentions📖autophagy-lysosomal-alzheimers50%
mentions📖Section 244: Advanced Autophagy Induction and TFEB Activatio50%
mentions📖Anterior Hypothalamic Area Neurons50%
mentions📖Cerebellar Purkinje Cells in Motor Coordination50%
mentions📖Cochlear Nuclei in Auditory Processing50%
mentions📖Cortical Basket Cells (Parvalbumin-Positive)50%
mentions📖Cortical Bipolar GABAergic Interneurons50%
mentions📖Cortical Chandelier Cells50%
mentions📖Dorsal Raphe Nucleus50%
mentions📖Flocculus Neurons50%
mentions📖GABA-C Receptor Neurons50%
mentions📖GABA Receptors in Neuronal Inhibition50%
mentions📖GABAergic Neuron Vulnerability50%
mentions📖GABA Transporter (GAT) Neurons50%
mentions📖Globus Pallidus External Segment Neurons50%
mentions📖Globus Pallidus Externus (GPe) Neurons50%
mentions📖Globus Pallidus Internal Segment (GPi) GABAergic Neurons50%
mentions📖Globus Pallidus Internus (GPi) Neurons50%
mentions📖Golgi Cells50%
mentions📖Habenula Neurons in Mood and Reward50%
mentions📖Habenula in Reward Prediction50%
mentions📖Habcnular Commissure Fibers50%
mentions📖Axo-Axonic Cells50%
mentions📖PV Interneurons (Hippocampus)50%
mentions📖Horizontal Limb of the Diagonal Band (HDB)50%
mentions📖Horizontal Limb of the Diagonal Band (HDB)50%
mentions📖Retinal Horizontal Cells50%
mentions📖Preoptic Area Sleep-Active Neurons50%
mentions📖Inferior Colliculus in Sound Localization50%
mentions📖Intercalated Amygdala Nucleus Neurons50%
mentions📖Interpeduncular Nucleus in Nicotine50%
mentions📖Interstitial Nucleus of Cajal50%
mentions📖Kölliker-Fuse Subnucleus50%
mentions📖Lateral Lemniscus Neurons50%
mentions📖Lateral Pericentral Nucleus of Inferior Colliculus Neurons50%
mentions📖Lateral Preoptic Area Neurons50%
mentions📖Laterodorsal Tegmental Nucleus50%
mentions📖Laterodorsal Tegmental Nucleus50%
mentions📖Laterodorsal Tegmental Nucleus GABAergic Neurons50%
mentions📖Lysosomal System in Neurons50%
mentions📖Main Olfactory Bulb (MOB) Neurons50%
mentions📖Medial Geniculate Body50%
mentions📖Medial Septal Cholinergic Neurons50%
mentions📖Median Preoptic Nucleus in Sleep Pressure50%
mentions📖Median Raphe Nucleus50%
mentions📖Olfactory Bulb Short-Axon Cells50%
mentions📖Pars Reticularis Nigral Neurons50%
mentions📖Parvalbumin Expressing Interneurons50%
mentions📖Preoptic Area GABAergic Neurons50%
mentions📖Purkinje Cell Axonal Terminals50%
mentions📖Renshaw Cells in Recurrent Inhibition50%
mentions📖Reticular Thalamic Nucleus GABAergic Neurons50%
mentions📖Reticularis Pontis Oralis Neurons50%
mentions📖Spinal Cord Lamina X Neurons50%
mentions📖Spinal Lamina II Neurons50%
mentions📖Cerebellar Stellate Cells50%
mentions📖Horizontal Cells of the Striatum50%
mentions📖Striatal Parvalbumin Interneurons50%
mentions📖Triangular Nucleus of the Septum (Tria) Neurons50%
mentions📖Tuberomammillary Nucleus Histaminergic Neurons50%
mentions📖Tuberomammillary Nucleus Expanded50%
mentions📖Ventral Pallidum (VP) Neurons50%
mentions📖Ventral Pallidum Neurons in Motivation50%
mentions📖Ventral Pallidum in Reward Valuation50%
mentions📖VTA GABAergic Neurons50%
mentions📖Ventral Tegmental Area GABAergic Neurons50%
mentions📖Ventrolateral Preoptic Area (VLPO) Neurons50%
mentions📖Ventrolateral Preoptic GABAergic Neurons50%
mentions📖Wernickes Area50%
mentions📖Lysosomal Enzyme Dysfunction Across Neurodegenerative Diseas50%
mentions📖entities50%
mentions📖Therapy Ideas50%
mentions📖YAP/TAZ Mechanoactivation Protocol for Neurodegeneration50%
mentions📖Autophagy Mechanisms50%
mentions📖mHTT Clearance Mechanisms in Huntington's Disease50%
mentions📖mTOR Signaling Pathway in Neurodegeneration50%
mentions📖Senescent Cell Clearance in Neurodegeneration50%
mentions📖Transcriptional Regulation in Neurodegeneration50%
mentions📖ubiquitin-proteasome-dysfunction-neurodegeneration50%
mentions📖Alpha-Synuclein Aggregation Inhibitors50%
mentions📖Ambroxol for Parkinson's Disease50%
mentions📖Baclofen50%
mentions📖Endosomal-Lysosomal Trafficking in CBS/PSP50%
mentions📖Hsp90 Inhibitors for Parkinson's Disease50%
mentions📖Neuroprotection Strategies in Neurodegeneration50%
mentions📖Progressive Supranuclear Palsy (PSP) Treatment Guide50%
mentions📖Section 193 Advanced Integrated Cell Stress Response Therapy50%
mentions📖USP30 Inhibitors for Parkinson's Disease50%
mentions📖MLCS Quantification in Parkinson's Disease50%
mentions📖Lipophagy Activation Therapy for Neurodegeneration50%
mentions📖Payload: Autophagy Proteostasis Dual Activation50%
mentions📖GBA Glucocerebrosidase Endolysosomal Pathway in Parkinson&#x50%
mentions📖Lysosome Dysfunction in Neurodegeneration50%
mentions📖mTOR Signaling in CBS/PSP50%
mentions📖Polyamine Metabolism Pathway in Neurodegeneration50%
mentions📖Protein Homeostasis in Neurodegeneration50%
mentions📖TBK1 Autophagy and Neuroinflammation ALS/FTD Causal Chain50%
mentions📖Autophagy Enhancers in Neurodegeneration50%
mentions📖Mitochondrial Therapies for Neurodegeneration50%
mentions📖spermidine-neurodegeneration50%
mentions📖Lysosomal Autophagy Neuron50%
mentions📖AMX0035 for Progressive Supranuclear Palsy50%
mentions📖ULK1/2 Kinase Modulation for Mitophagy Induction in Neurodeg50%
mentions📖AMPK Signaling in Parkinson's Disease50%
mentions📖ATP13A2 Lysosomal Dysfunction Parkinson's Disease Causa50%
mentions📖Autophagy Types in Neurodegeneration50%
mentions📖Endolysosomal Pathway in Alzheimer's Disease50%
mentions📖Lysosomal Dysfunction in Neurodegeneration50%
mentions📖Lysosomal Storage Diseases50%
mentions📖mTOR Signaling in Neurodegeneration50%
mentions📖mTOR Signaling Pathway in Neurodegeneration50%
mentions📖VCP→TDP-43→ALS/FTD Causal Chain50%
mentions📖Protein Homeostasis Therapies50%
mentions📖Section 206: Advanced Heat Shock Protein and Molecular Chape50%
mentions📖Lipid Droplet-Lysosome Axis Therapeutic Testing in Parkinson50%
mentions📖Mitochondria-Lysosome Contact Sites Dysfunction in Parkinson50%
mentions📖Lysosomal Dysfunction Therapeutics Investment Landscape50%
mentions📖Endolysosomal Trafficking Defects in Neurodegeneration50%
mentions📖Membrane Trafficking Pathway Dysfunction in Neurodegeneratio50%
mentions📖Trehalose for Neurodegenerative Diseases50%
mentions📖Autophagy and Mitophagy Therapeutics Investment Landscape50%
mentions📖ATP13A2/PARK9 Lysosomal Function Therapies for Parkinson50%
mentions📖CBS Autophagy-Lysosomal Pathway Dysfunction50%
mentions📖mHTT Clearance Mechanisms in Huntington's Disease50%
mentions📖mTOR Signaling Dysregulation in Progressive Supranuclear Pal50%
mentions📖Target Family Consolidation Analysis — Cross-Disease Therape50%
mentions📖Proteostasis Network Enhancement for CBS/PSP50%
mentions📖Autophagy-Lysosomal Pathway Dysfunction in Corticobasal Synd50%
mentions📖Autophagy-Lysosomal Dysfunction in Neurodegenerative Disease50%
mentions📖Macroautophagy Dysfunction Validation Framework in Parkinson50%
mentions📖Protein Kinase C (PKC) Signaling in Parkinson's Disease50%
mentions📖AKT (Protein Kinase B) Neurons44%
mentions📖Autophagy-Deficient Neurons in Neurodegeneration44%
mentions📖Dendritic Spines in Neurodegeneration44%
mentions📖GDNF (Glial Cell Line-Derived Neurotrophic Factor) Neurons44%
mentions📖mTOR (Mammalian Target of Rapamycin) Neurons44%
mentions📖Synaptic Boutons44%
mentions📖AD RNA-Based Therapeutics Companies44%
mentions📖Nucleus Basalis of Meynert44%
mentions📖Autophagy Enhancement Drug Screening for Neurodegeneration44%
mentions📖Priority Research Areas for Neurodegenerative Diseases44%
mentions📖Mechanistic Proposal: Pathologic synergy: The pathologic agg44%
mentions📖LRRK2 Kinase Modulation for Pre-Symptomatic Carriers44%
mentions📖AAV-Delivered RNA Targeting Therapy for Neurodegeneration44%
mentions📖AMPK Agonist Therapy for Neurodegeneration44%
mentions📖Cathepsin D Activation Therapy for Lysosomal Proteostasis44%
mentions📖FUS Protein-Targeting Therapy for ALS/FTD44%
mentions📖HSP70 Inducer Therapies for Neurodegeneration44%
mentions📖mRNA-Encoded Intrabody for Alpha-Synuclein Clearance44%
mentions📖Perivascular Space Drainage Enhancement Therapy44%
mentions📖Stress Granule Modulation Therapy44%
mentions📖TMEM163 Lysosomal Restoration Therapy for Neurodegeneration44%
mentions📖VPS13 Lipid Transport and Organelle Contact Site Modulation 44%
mentions📖4R Tauopathy Therapeutics — Investment Landscape Analysis44%
mentions📖Adult Neurogenesis Therapeutics Investment Landscape44%
mentions📖Anti-Tau Therapeutics: Investment Landscape Analysis44%
mentions📖ER Stress & UPR Therapeutics Investment Landscape44%
mentions📖GLP-1 Receptor Agonist Therapeutics Investment Landscape44%
mentions📖Heat Shock Protein (HSP) Therapeutics for Neurodegeneration44%
mentions📖Microbiome-Based Therapeutics for Neurodegeneration: Investm44%
mentions📖Mitochondrial Therapeutics Investment Landscape44%
mentions📖Peptide Therapeutics for Neurodegeneration — Investment Land44%
mentions📖Protein Aggregation Inhibitors for Neurodegeneration — Inves44%
mentions📖RNA Therapeutics: Investment Landscape Analysis44%
mentions📖Senolytic Therapeutics: Investment Landscape Analysis44%
mentions📖TDP-43 Proteinopathy in ALS44%
mentions📖AMPK Signaling in Neurodegeneration44%
mentions📖AMPK Signaling Pathway44%
mentions📖Anti-Amyloid Therapeutics: Successes, Failures, and Lessons 44%
mentions📖Autophagy Modulation with Bioactive Compounds for Neurodegen44%
mentions📖Autophagy Dysfunction in Progressive Supranuclear Palsy44%
mentions📖Autophagy-Lysosome Pathway in Neurodegeneration44%
mentions📖CBS Lysosomal Dysfunction and Autophagy Impairment44%
mentions📖Chaperone-Mediated Autophagy in Neurodegeneration44%
mentions📖Cross-Disease Mechanistic Convergence Investment Ranking44%
mentions📖Emerging Therapeutic Directions 2025-202644%
mentions📖Hippo Pathway in Neurodegeneration44%
mentions📖Hippo Signaling in 4R-Tauopathies44%
mentions📖Insulin Signaling in Parkinson's Disease44%
mentions📖LRRK2→Kinase→Autophagy→PD Causal Chain44%
mentions📖Lysosomal Dysfunction in Progressive Supranuclear Palsy44%
mentions📖MLCS Research Methods in iPSC-Derived Dopaminergic Neurons44%
mentions📖Mutant Huntingtin Aggregation Pathway44%
mentions📖Neuroresilience Mechanisms and Evidence44%
mentions📖p70S6K/mTORC1 Signaling Pathway in Neurodegeneration44%
mentions📖PD Cure Roadmap44%
mentions📖PINK1/PARKIN Mitophagy Activators for Parkinson's Disea44%
mentions📖pink1-parkin-mitophagy-pd-causal-chain44%
mentions📖Progranulin Haploinsufficiency in Frontotemporal Dementia44%
mentions📖Proteostasis Network in Neurodegeneration44%
mentions📖Stress Granule Homeostasis in ALS/FTD44%
mentions📖tdp-43-fus-rna-proteinopathy-comparison44%
mentions📖TDP-43 RNA Granule Pathway44%
mentions📖Therapeutic Approach Evidence Rankings44%
mentions📖Transcriptional Dysregulation in Neurodegeneration44%
mentions📖Ubiquitin-Proteasome System Dysfunction in Parkinson's 44%
mentions📖Denali Therapeutics44%
mentions📖Digital Therapeutics for Neurodegenerative Diseases44%
mentions📖P300 Brain-Computer Interface44%
mentions📖RNA Editing Therapeutics44%
mentions📖Stem Cell Therapies for Neurodegenerative Diseases44%
mentions📖Alpha-Synuclein Reduction Therapies for Neurodegenerative Di44%
mentions📖ampk-activators44%
mentions📖AMPK Activators for Neurodegeneration44%
mentions📖Anti-Tau Therapeutics44%
mentions📖Exercise and Physical Activity for Neuroprotection44%
mentions📖Exosome-Based Therapeutics for Neurodegenerative Diseases44%
mentions📖LLPS Modulator Therapy44%
mentions📖Longevity and Rejuvenation Therapies Landscape44%
mentions📖Mitochondrial Therapeutics44%