🧫

Trehalose-induced lysosomal changes and TFEB activation

active
experiment Created: 2026-04-06T12:29:04 By: experiment_extractor Quality: 90% ✓ SciDEX ID: experiment-exp-b939a41c-0445-4658-bdaf-1
🧫 Experiment Protocol Cell Biologyproposed
Related Entities
TFEB
Metadata
_origin{'url': None, 'type': 'internal', 'tracked_at': '2026-04-06T12:29:04.358509'}
p_valueNone
protocolCells treated with trehalose, followed by immunofluorescence analysis for TFEB localization and lysosomal markers, biochemical assays for PPP3/calcineurin activity, and measurement of lysosomal membra
effect_sizeNone
sample_sizeNone
source_pmid30335591
extracted_at2026-04-06T05:29:04.356050
_schema_version1
experiment_typecell_biology
success_criteriaSignificant TFEB nuclear translocation, lysosomal enlargement, and increased autophagy gene expression
expected_outcomesTrehalose treatment should induce lysosomal changes leading to TFEB activation and autophagy upregulation
statistical_methodsNone
📊 Evidence Profile Foundational
Evidence Balance
+0%
Certainty
100%
Debates
0
Incoming
299
Outgoing
25
0 supporting 0 contradicting 0 neutral
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