Microglia-Derived Extracellular Vesicle Engineering for Targeted Mitochondrial Delivery

graph TD
    A["Microglia"] --> B["Natural EV Secretion"]
    B --> C["Extracellular Vesicles"]

    D["Engineering EVs"] --> E["RAB27A Overexpression"]
    E --> F["Enhanced EV Production"]

    D --> G["LAMP2B Surface Targeting"]
    G --> H["Neuron-Specific Ligands"]
    H --> I["Targeted EV Delivery"]

    D --> J["Load Healthy Mitochondria"]
    J --> K["Mito-Enriched EVs"]

    F --> L["Engineered Microglia-EVs"]
    I --> L
    K --> L

    L --> M["Targeted Delivery to Damaged Neurons"]

    N["Damaged Neurons"] --> O["Mitochondrial Dysfunction"]
    O --> P["Energy Failure"]

    M --> Q["Mito Integration into Neurons"]
    Q --> R["Restored Bioenergetics"]
    R --> S["Neuronal Rescue"]

    T["Advantages"] --> U["Cross BBB via EVs"]
    T --> V["Cell-Type-Specific Targeting"]
    T --> W["Scalable Production"]

    S --> X["Targeted Mitochondrial Therapy"]

    style A fill:#264653,stroke:#ffd54f,color:#e0e0e0
    style D fill:#1a3a4a,stroke:#4fc3f7,color:#e0e0e0
    style M fill:#1a3a2a,stroke:#81c784,color:#e0e0e0
    style X fill:#2a3a1a,stroke:#c5e1a5,color:#e0e0e0