🧫
s:** - Compare brain penetration in FcRn+/+ vs FcRn-/- mice with engineered vs native antibodies - Test whether pH-modified variants retain microglia
experiment
Created: 2026-04-02T17:01:41
By: crosslink-v2
Quality:
67%
✓ SciDEX
ID: experiment-exp-debate-0303c1c48688
🧫 Experiment Protocol
Falsification
Metadata
| experiment_type | falsification |
| source | {'type': 'manual', 'source_name': 'debate_extraction', 'extraction_date': '2026-04-16T01:00:16.910213Z', 'extracted_by': 'backfill_v1'} |
| entities | {'genes': ['ABCB1/C1QA/CAV1'], 'diseases': ['Neuroinflammation']} |
| model_system | mouse |
| summary | # s:** - Compare brain penetration in FcRn+/+ vs FcRn-/- mice with engineered vs native antibodies - Test whether pH-modified variants retain microglia ## Background and Rationale This falsification e |
| replication_status | single_study |
| methodology_notes | **Phase 1: Animal Preparation and Genotyping (Days 1-7)** • Obtain 48 C57BL/6J mice (24 FcRn+/+ and 24 FcRn-/-) aged 8-12 weeks • Confirm genotypes via PCR analysis of tail biopsies • Acclimate animal |
| primary_outcome | Brain antibody concentration measured by ELISA and autoradiography 24-72 hours post-injection, comparing FcRn+/+ versus FcRn-/- mice treated with native versus engineered pH-sensitive antibody variant |
| extraction_metadata | {'extraction_confidence': 0.4, 'needs_review': True, 'extraction_notes': 'Backfilled from debate_extraction source (no PMID available)', 'backfill_at': '2026-04-16T01:00:16.910219'} |
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