Synthetic Biology BBB Endothelial Cell Reprogramming

**Molecular Mechanism and Rationale** The blood-brain barrier (BBB) represents one of the most formidable obstacles in neurotherapeutics, with its tightly regulated endothelial cells severely limiting drug penetration into the central nervous system. This synthetic biology approach targets the fundamental transcytosis machinery of brain microvascular endothelial cells through precise genetic reprogramming of four critical membrane transport proteins. The molecular strategy exploits the natural ...