biomarker 3,114 words KG: PD 2026-03-24
kind:biomarkersection:biomarkersstate:published
Contents

Alpha-Synuclein Seed Kinetic Staging in Parkinson's Disease

🔬 Protein Info
Gene SymbolPD
Reaction mixtureContains recombinant alpha-synuclein monomer (substrate), Thioflavin T (fluorescent dye), and NaCl
Cycling conditionsAlternating periods of shaking (1 min) and rest (1 min) at 30°C for 30-100 hours
DetectionThioflavin T fluorescence measured every 15-30 minutes
Positive responseSeed-induced aggregation causes increased fluorescence
SensitivityCan detect as few as 10-100 aggregated alpha-synuclein seeds[@okuzumi2023]
Associated DiseasesDEPRESSION, NEURODEGENERATION, T2DM
Known Drugs/CompoundsEPS
KG Connections59 knowledge graph edges
DatabasesGeneCardsUniProtNCBI GeneHPASTRING

Knowledge Graph

Related Hypotheses (13)

Gut Microbiome Remodeling to Prevent Systemic NLRP3 Priming
Score: 0.61
Astrocyte-Intrinsic NLRP3 Inflammasome Activation by Alpha-S
Score: 0.60
Microbial Inflammasome Priming Prevention
Score: 0.58
Epigenetic Memory Reprogramming via CRISPRa-Mediated Chromat
Score: 0.52
Conditional CRISPR Kill Switches for Aberrant Protein Cleara
Score: 0.45

Related Analyses (16)

Digital biomarkers and AI-driven early detection of neurodeg
neurodegeneration · archived
What are the mechanisms by which gut microbiome dysbiosis in
neurodegeneration · archived
TREM2 agonism vs antagonism in DAM microglia
neurodegeneration · failed
Selective vulnerability of entorhinal cortex layer II neuron
neurodegeneration · archived
TDP-43 phase separation therapeutics for ALS-FTD
neurodegeneration · archived

Related Experiments (30)

AAV Serotype Comparison for LRRK2 Knockdown in PD Gene Thera
validation · proposed · Score: 0.40
AAV-LRRK2 IND-Enabling Study Design
clinical · proposed · Score: 0.40
AAV Serotype Comparison for LRRK2 Knockdown in PD
validation · proposed · Score: 0.40
Alpha-Synuclein SAA Kinetics Study — Biological Staging Back
clinical · proposed · Score: 0.40
Alpha-Synuclein Aggregation Triggers — Sporadic PD Initiatio
clinical · proposed · Score: 0.40

See Also (11)

LRRK2 G2019S Transgenic Mouse Model
model · KG edge: causes
Age-Sensitive Cortical Vulnerability in Parkinson's Dis
mechanism · KG edge: associated_with
MCL1 Gene - Myeloid Cell Leukemia 1
gene · KG edge: implicated_in
IDH1 Gene - Isocitrate Dehydrogenase 1
gene · KG edge: implicated_in
eef1a1
gene · KG edge: implicated_in

Knowledge Graph (12 edges)

PD causes NEURODEGENERATION
PD causes DOPAMINERGIC_NEURONS
PD causes neurodegeneration
PD contributes_to synucleinopathies
PD associated_with DEPRESSION
PD associated_with T2DM
NEUROINFLAMMATION contributes_to PD
NEUROTROPHINS treats PD
AGE associated_with PD
SNCA causes PD