Microbial Inflammasome Priming Prevention

graph TD
    A["Microbial PAMPs<br/>LPS and Bacterial Components"] --> B["TLR4 and PRR<br/>Pattern Recognition"]
    B -->|"Signal 1"| C["NF-kappaB Nuclear<br/>Translocation"]
    C --> D["Pro-IL1B and Pro-IL18<br/>Transcription"]
    
    E["ATP and Danger Signals<br/>Extracellular"] -->|"Signal 2"| F["P2X7 Receptor<br/>Activation"]
    F --> G["Potassium Efflux<br/>and ROS Generation"]
    G --> H["NLRP3 Protein<br/>Conformational Change"]
    
    D --> I["Cytoplasmic Pro-IL1B<br/>Accumulation"]
    H --> J["PYCARD ASC Adapter<br/>Recruitment"]
    J --> K["Pro-CASP1<br/>Oligomerization"]
    K --> L["Active Caspase-1<br/>Formation"]
    
    I --> L
    L -->|"Proteolytic Cleavage"| M["Mature IL1B<br/>Release"]
    L --> N["Gasdermin D<br/>Pore Formation"]
    
    M --> O["Neuroinflammatory<br/>Cascade Activation"]
    N --> P["Pyroptotic Cell Death<br/>and DAMP Release"]
    P --> Q["Microglial Activation<br/>and Proliferation"]
    Q --> R["Synaptic Dysfunction<br/>and Neurodegeneration"]
    
    S["NLRP3 Inhibitors<br/>MCC950 Treatment"] -->|"Therapeutic Block"| H
    T["Caspase-1 Inhibitors<br/>VX-765 Compounds"] --> L
    U["IL1B Antagonists<br/>Anakinra Therapy"] --> O
    
    O --> R
    
    classDef normal fill:#4fc3f7,stroke:#2196f3,color:#0d0d1a
    classDef therapeutic fill:#81c784,stroke:#4caf50,color:#0d0d1a
    classDef pathology fill:#ef5350,stroke:#f44336,color:#0d0d1a
    classDef outcome fill:#ffd54f,stroke:#ff9800,color:#0d0d1a
    classDef molecular fill:#ce93d8,stroke:#9c27b0,color:#0d0d1a
    
    class A,B,E,F normal
    class H,J,K,L,I,M,N molecular
    class O,Q,P pathology
    class R outcome
    class S,T,U therapeutic
    class C,D,G normal